Thursday, 11 August 2011

Syllabus of B.E. (Biotechnology) 3rd to 8th semesterOf Punjab University, Chandigarh

SCHEME OF EXAMINATION OF B.E. BIOTECHNOLOGY (2011-12)
Second Year - Third Semester
Scheme Scheme of Teaching of Examination
Theory/ Practical
Sub Code Subject
L T P Hrs Credits Sess. Univ.
Exam.
Total
BIO301 Concepts in Bio-
Technology-I
4 0 0 4 4 50 50 100
BIO351 Concepts in Bio-
Technology-I
(Prac.)
0 0 2 2 1 50 0 50
BIO302 Microbiology 4 0 0 4 4 50 50 100
BIO352 Microbiology (Prac.) 0 0 3 3 2 50 0 50
BIO303 Biochemistry 4 0 0 4 4 50 50 100
BIO353 Biochemistry (Prac.)
0 0 3 3 2 50 0 50
BIO304 Cell & Molecular
Biology
4 0 0 4 4 50 50 100
BIO354 Cell & Molecular
Biology
0 0 2 2 1 50 0 50
BIO305 Material and Energy
Balance Calculations
3 1 0 4 4 50 50 100
AS301
Mathematics-III
3 1 0 4 4 50 50 100
Total 22 2 10 34 30 500 300 800

SCHEME OF EXAMINATION OF B.E. BIOTECHNOLOGY (2011-12)
Second Year - Fourth Semester
Scheme Scheme of Teaching of Examination
Theory/ Practical
Sub
Code
Subject
L T P Hrs Credits Sess. Univ.
Exam.
Total
BIO401 Concepts in
Biotechnology-II
4 0 0 4 4 50 50 100
BIO451 Concepts in
Biotechnology-II
(Prac.)
0 0 2 2 1 50 0 50
BIO402 Process
Thermodynamics
4 0 0 4 4 50 50 100
BIO403 Chemical Reaction
Engineering
4 0 0 4 4 50 50 100
BIO453 Chemical Reaction
Engineering (Prac.)
0 0 2 2 1 50 0 50
BIO404 Industrial
Microbiology
4 0 0 4 4 50 50 100
BIO454 Industrial
Microbiology (Prac.)
0 0 2 2 1 50 0 50
BIO405 Immunology &
Immuno-technology
4 0 0 4 4 50 50 100
BIO455 Immunology &
Immuno-technology
(Prac.)
0 0 2 2 1 50 0 50
BIO406 Legal Laws and IPR 4 0 0 4 4 50 50 100
Total
24 0 8 32 28 500 300 800
SCHEME OF EXAMINATION OF B.E. BIOTECHNOLOGY
Third Year - Fifth Semester
Theory Practical
Theory
Paper
Code
Paper Title Hours/
Week
Marks
Uni.
Exam
Int.
Ass.
Hours/
Week
Marks
Uni.
Exam
Int.
Ass.
Practical
Paper
Code
BIO501 Enzyme Engineering &
Technology
4+0 100 50 2 50 50 BIO551
BIO502 Bio-Process Engineering 4+0 100 50 2 50 50 BIO552
BIO503 Animal Cell Culture &
Biotechnology
4+0 100 50 2 50 50 BIO553
BIO504 Transport Phenomena 4+0 100 50 2 50 50 BIO554
BIO505 Bio-Process Technology 4+0 100 50 -- -- -- --
BIO506
BIO507
Bioinstrumentation
Training of 8 weeks after 4th
semester exams
4+0 100 50 -- -- --
50
--
Grand Total: 1350
24 600 300 08 200 250
Third Year - Sixth Semester
Theory Practical
Theory
Paper
Code
Paper Title Hours/
week
Marks
Uni.
Exam
Int.
Ass.
Hours/
Week
Marks
Uni.
Exam
Int.
Ass.
Practical
Paper
Code
BIO601 Recombinant DNA
Technology
4+0 100 50 2 50 50 BIO651
BIO602 Operation Research 3+1 100 50 2 50 50 BIO652
BIO603 Introduction to Bio-
Informatics
4+0 100 50 2 50 50 BIO653
BIO604 Bioreactor Design and
Operation
4+0 100 50 -- -- -- --
BIO605 Down Stream Processing 4+0 100 50 3 50 50 BIO655
BIO606 Biomaterials 4+0 100 50 -- -- -- --
Grand Total: 1300
24 600 300 9 200 200
SCHEME OF EXAMINATION OF B.E. BIOTECHNOLOGY
Fourth Year - Seventh Semester
Theory Practical
Theory
Paper
Code
Paper Title Hours/
week
Marks
Uni.
Exam
Int.
Ass.
Hours/
Week
Marks
Uni.
Exam
Int.
Ass.
Practical
Paper
Code
BIO701 Environmental
Biotechnology
4+0 100 50 2 50 50 BIO751
BIO702 Food Biotechnology 4+0 100 50 2 50 50 BIO752
BIO703 Plant Tissue Culture 4+0 100 50
BIO704 Bioanalytical Techniques 4+0 100 50 2 50 50 BIO754
BIO705 Minor Project -- -- -- 5 75 75 --
BIO706 Training of 4 weeks after
6th semester exams
50
Grand Total: 1100
16 400 200 11 225 275
SCHEME OF EXAMINATION OF B.E. BIOTECHNOLOGY
Fourth Year - Eighth Semester
Theory Practical
Theory
Paper
Code
Paper Title Hours/
Week
Marks
Uni.
Exam
Int.
Ass.
Hours/
Week
Marks
Uni.
Exam
Int.
Ass.
Practical
Paper
Code
BIO801 Major Project -- -- -- 5 150 150 --
BIO802
BIO803
BIO804
Enzyme catalyzed
organic synthesis
Project management and
Entrepreneurship
Modeling and Simulation
of Bio-Processes
4+0
4+0
4+0
100
100
100
50
50
50
3
3
50
50
50
50
BIO852
BIO854
BIO805 Elective – I * 4+0 100 50 -- -- -- --
Option 1
Grand Total: 1100
16 400 200 11 250 250
Option 2 BIO 860 Industrial Training - 650 450
Grand Total: 1100
Options in Elective - I*
1. Nanobiotechnology
2. Microbial Biodiversity
Conditions for choosing Option 1 or Option 2 in 8th Semester:
A student may opt for either Option 1 or one semester training (Option 2) in lieu of
subjects of 8th Semester (option 1). The marks for six months training will be equal to
the total marks of 8th Semester study. A student can opt for six semester training under
following conditions: -
a. The student got selected for job in campus placement and the employer is
willing to take that student for the training.
b. The student got offer of pursuing training from reputed government research
organization/govt. sponsored projects/govt. research institution provided that
student should not be paying any money to get trained. For pursuing this training
student needs the prior approval from the Coordinator of the respective branch.
SYLLABUS
B.E. IN BIOTECHNOLOGY
THIRD SEMESTER
Paper Title: Concepts in Biotechnology-I (Theory)
Paper Code: BIO 301 Max. Marks 50 Credits: 4
Course Duration: 45 Lectures of one hour each.
.Note for the Paper setter: The question paper should be divided into Section A and
Section B Total of 8 questions, 4 from section A and 4 from section B are to be set.. The
students will be required to attempt 5 questions selecting at least 2 from each section.
SECTION-A
1. Introduction to Biotechnology: Historical perspectives, Emergence of modern
biotechnology, Branches / scope of biotechnology
2. Biological systems in biotechnology: (a) Prokaryotic cell structure and systems:
E.coli, Bacillus
(b) Eukaryotic cell structure and systems: Saccharomyces, mammalian and nonmammalian
cells in culture.
1. Basic techniques in biotechnology: Centrifugation : Principle, types, application,
Electrophoresis : Principle, support media, protein and DNA electrophoresis, SDSPAGE,
Chromatography: Principle, types, application, Lyophilization : Principle,
mechanism, application, Basic microscopy : Principle, various types of microscopes,
introduction to electron microscopy, Radioisotopy : Basic Concepts, Various types of
radio isotopes used in biology.
SECTION- B
2. Basic genetics : Mendelian inheritance, physical basis of inheritance, gene
interactions., Bacterial and viral genetic systems., Genomic and mitochondrial
DNA, C value paradox, cot curve, chromosome walking, Overview of DNA
replication, genetic code, transcription, translation, Cytoplasmic inheritance,
nucleo-cytoplasmic interactions.
3. Genetic manipulations: Concepts, enzymes involved in it, vectors, PCR, DNA
sequencing,, construction and screening of a genomic library and c-DNA library
containing foreign DNA sequences. Production of recombinant proteins.
6. Mutagenesis and protein engineering: Basic concepts only
BOOKS RECOMMENDED
a. Techniques used in Bioproduct Analysis-Biotol Series(I-IV), 1992, Buterworth
Heineman,UK, 1992.
b. Basic Biotechnology By B..D. Singh (2004),Kalyani Pub. New Delhi
c. Membrane Separation in Biotech by McGregor, C.W., 1986, Marcel Dekker Inc.,
New York.
b. Separation, Recovery and Purification in Biotech by Juan H., and Sengo, A.A., 1985,
American Chem. Society, Washington.
c. Lab. Techniques in Biochemistry and Molecular Biology, 1984, Elsevier, New York.
e. Physical Biochemistry,1982, Friefelder Freeman and Co., New York.
Paper Title: Concepts in Biotechnology-I (Practical)
Paper Code: BIO 351 Max. Marks 50 Credits: 1
Practicals:
1. Introduction to instrumentation: centrifuges, autoclaves,
spectrophotometers, microscopes, laminar hoods, incubators, CO2
incubators, lyophilizer etc.
2. Polyacrylamide gel electrophoresis for proteins.
3. Use of microscopes: compound microscope, fluorescent
microscope, inverted microscope and visit to E.M. lab.
4. Use of Geiger muller counter.
Paper Title: Microbiology (Theory)
Paper Code: BIO 302 Max. Marks 50 Credits: 4
Course Duration: 45 Lectures of one hour each.
Note for the Paper setter: The question paper should be divided into Section A and
Section B Total of 8 questions, 4 from section A and 4 from section B are to be set.. The
students will be required to attempt 5 questions selecting at least 2 from each section.
SECTION-A
1. History of microbiology, Germ theory, Disciplines of microbiology,
2. Structure of microbes (bacteria, algae, fungi and viruses)
3. Microbial taxonomy including modern approaches of taxonomy such as DNA
taxonomy and numerical taxonomy, different groups in bacteria.
4. Microbial growth : synchronous & asynchronous, pure cultures, growth inhibitory
substances.
SECTION-B
5. Microbial genetics, transformation, conjugation & transduction mutations.
6. Microbial Metabolisms :Nutrition, media and methods, effects of environment,
metabolic products of industrial importance, metabolic pathways amphicatabolic and
biosynthetic.
7 Microbial ecology (with particular reference to C, N cycles)
8. Microbes as pathogens : Important microbial pathogens, toxins, mode of action of
toxins
Books Recommended:
1. Microbiology by Pelczar M.J. and Chan. E.C.S.(Jr.), Reprint,2008, Tata
McGraw Hill Pub. Co., New Delhi.
2. Medical Microbiology by Bernard, D.D., Dulbecco, R., Eisen, H.N., and
Ginsbery, H.S., 1990, Harper and Row, New York.
3. Industrial Microbiology by Waites M.J. et al., 2001, Blackwell Science Ltd.,
London.
4. Instant Notes in Microbiology by Nicklin J. et al., 2002, BIOS Scientific
publishers, Ltd., U.K.
5. Microbiology: An Introduction by Tortora Benjamin/Cummings Pub. Co.,
10th edition, 2009.
Paper Title: Microbiology (Practical)
Paper Code: BIO 352 Max. Marks -50 Credits: 2
Practicals:
1. Experiments on isolation-spread plate, enrichment culture; staining and
measurement of microbes; effect of environment on microbial growth;
lyophilisation.
2. Testing of microbiological qualities of milk and water; growth and production
formation-batch culture, enzyme production, assay of enzymes.
3. Microbial assay of antibiotics.
4. Studies on auxotrophs
5. Detection of an enzyme production by a microbe
6. Determination of molecular weight of an enzyme.
Paper Title: Biochemistry (Theory)
Paper Code: BIO 303 Max. Marks 100 Credits: 4
Course Duration: 45 Lectures of one hour each.
Note for the Paper setter: The question paper should be divided into Section A and
Section B Total of 8 questions, 4 from section A and 4 from section B are to be set.. The
students will be required to attempt 5 questions selecting at least 2 from each section.
SECTION-A
1. Chemistry and properties of following Biomolecules:
a)amino acids b) proteins c) carbohydrates d) lipids e) nucleic
acids, f) water soluble vitamins.
2. Chemical Bonds: Covalent bonds, co-ordinate bonds , hydrogen bonds,
Vander waal's forces , hydrophobic interactions , ionic bonds , dipole
interactions.
3. Proteins: Primary, secondary, tertiary and quaternary structures, methods
for isolation and purification of proteins, protein analysis and amino acid
sequence determination.
4. Carbohydrate metabolism: Glycolysis, glycogenolysis, glycogenesis and their
regulation, citric acid cycle.
SECTION-B
5. Fat metabolism: Oxidation of fatty acids, synthesis of fatty acids (fatty acid
synthase complex), ketone bodies.
6. Amino acid metabolism: General pathways of amino acid metabolism ,
transamination, decarboxylation , deamination , Urea cycle.
7. Nucleic acid metabolism: Biosynthesis of purines and pyrimidine nucleotides,
biosynthesis of deoxyribonucleotides , their regulation , catabolism.
8. Mitrochondria: Structure of mitochondria, organization of respiratory chain,
oxidative phosphorylation .
10. Plant and microbial biochemistry- Photosynthesis and Nitrogen fixation
Books Recommended:
a) Principles of Biochemistry, Lehninger, A.L., Nelson, DL and Cox, M. M. 4th revised
edition, 2008, Worth Publishers, New York
b) Harper's Biochemistry by Murray, R.K, 2006, 28thedition, 2008, Mc Graw – Hill,
America.
c) Biochemistry by Zubay, G., 4thedition,1999, W..C.Brown Publishers, Oxford, England.
d). Biochemistry by Stryer, L., 2006, 6th edition W.H. Freeman and Company, New
York.
e) Biochemistry by Voet, D and Voet J.G., 2006,3rd edition, John Wiley and Sons, Inc.
New York.
f) Outlines of biochemistry by Conn, E.C. and Stumph, P.K. 5th edition, 2006, John
Wiley & Sons.
Paper Title: Biochemistry (Practical)
Paper Code: BIO353 Max. Marks 50 Credits: 2
Practicals :
Qualitative tests of carbohydrates (monosaccharides, disaccharides,
polysaccharides, reducing and non reducing sugars etc.), proteins and amino acids, paper
chromatography of amino acids or sugars. Estimation of proteins ( by Biuret method and
Lowry method) carbohydrates, DNA, RNA, Cholesterol, lipids etc by
spectrophotometry.
Paper Title: Cell and Molecular Biology (Theory)
Paper code: BIO 304 Max. Marks: 50 Credits: 4
Course Duration: 45 Lectures
Note for the Paper setter: The question paper should be divided into Section A and
Section B Total of 8 questions, 4 from section A and 4 from section B are to be set.. The
students will be required to attempt 5 questions selecting at least 2 from each section.
SECTION-A
Membrane structure and function, cytoskeleton, ECM and its role in cell behavior regulation.
(5)
Chromosomes and organization of DNA.
(3)
DNA Replication in Prokaryotes and Eukaryotes: Basic process, enzymes involved in replication.
(6)
DNA Recombination : A brief introduction to molecular mechanisms in Prokaryotes and
Eukaryote(4)
Insertion elements and Transposons: Background, types of IS elements and transposons.
(4)
SECTION-B
Transcription in Prokaryotes and Eukaryotes: Mechanism, factors in prokaryotes and eukaryotes,
regulatory mechanisms like substitution of sigma factor, Lac operon in prokaryotes, regulation of
gene expression in eukaryotes, post-transcriptional changes in eukaryotes, RNA editing.
(8)
Translation in Prokaryotic and Eukaryotic cells: stages, molecules involved differential
translation. (6)
Cell Cycle and its Regulation: Phases of cell cycle, cell cycle check points, brief introduction to
factors involved in cell cycle regulation. (4)
Signal Transduction: Signal transduction through receptor interacting with G-protein/ receptor
with tyrosine kinase activity, role of second messengers like c-AMP, Inositol- tri- phosphate,
Diacyl glycerol, Ca2+ ions and mechanisms. (4)
Role of molecular Biology in Molecular Medicine. (1)
Books Recommended :
Latchman,D., 2006 : Basic Molecular and Cell Biology(2009). Black Well pub.,
3rd edition.
Lewin, B., 2003 : Gene VIII, Oxford University Press, New York.
Stansfield, W. D., Colome, J.S. : Schaum’s Outline of Theory and Problems of Molecular
and Cano, R. J., 2004 and Cell Biology, Tata McGraw Hill Edition, 1996, TMH.
Glick, B.R. and Pasternak, J. J., : Molecular Biotechnology, Principles and applications of
2004 recombinant DNA, Second edition, American Society for
Microbiology Press, Washington D.C.
Kendrew, S.J., 1994 : The Encyclopedia of Molecular Biology, Blackwell, U.K.
Paper Title: Cell and Molecular Biology (Practical)
Paper Code : BIO 354 Max. Marks : 50 Credits: 4
1. Isolation of plasmid DNA from E.coli.
2. Isolation of Genomic DNA from E.coli cells.
3. Agarose Gel Electrophoresis.
4. Preparation of CaCl2 competent cells.
5. Preparation of electrocompetant cells.
6. Transformation of E.coli cells with the plasmid DNA by using CaCl2.
7. Transformation of E.coli cells with the plasmid DNA by using electroporator.
Paper Title: Engineering Mathematics – III
Paper Code: BIO/AS -306 Maximum Marks: 100 Credits: 4
Course Duration: 45 lectures of one hour each.
Note for the Paper setter: The question paper should be divided into Section A and
Section B Total of 8 questions, 4 from section A and 4 from section B are to be set.. The
students will be required to attempt 5 questions selecting at least 2 from each section
SECTION- A
Sequences and Series: Sequences, Limits of sequences, Infinite series, series of positive
terms, Integral test, Comparison test, Ratio test, Root test. Alternating series, Absolute
and Conditional Convergence, Leibnitz test. Power series: radius of convergence of
power series, Taylor’s and Maclaurin’s Series, Formulae for remainder term in Taylor
and Maclaurin series, Error estimates. (Scope as in Chapter 8, Sections 8.1 – 8.10 of
Reference 2). (8 Lectures)
Linear Algebra: Concept of linear independence and dependence, Rank of a matrix:
Row – Echelon form, System of linear equations: Condition for consistency of system of
linear equations, Solution by Gauss elimination method. Inverse of a matrix: Gauss –
Jordan elimination method (Scope as in Chapter 6, Sections 6.3 – 6.5, 6.7 of Reference
1). (7 Lectures)
Eigen values, eigen vectors, Cayley – Hamilton theorem (statement only). Similarity of
matrices, Basis of eigenvectors, diagonalization (Scope as in Chapter 7, Sections 7.1, 7.5
of Reference 1). (7 Lectures)
SECTION-B
Complex Functions: Definition of a Complex Function, Concept of continuity and
differentiability of a complex function, Cauchy – Riemann equations, necessary and
sufficient conditions for differentiability (Statement only). Study of complex functions:
Exponential function, Trigonometric functions, Hyperbolic functions, real and imaginary
part of trigonometric and hyperbolic functions, Logarithmic functions of a complex
variable, complex exponents (Scope as in Chapter 12, Sections 12.3 – 12.4, 12.6 – 12.8 of
Reference 1).
(8 Lectures)
Laurent Series of function of complex variable, Singularities and Zeros, Residues at
simple poles and Residue at a pole of any order, Residue Theorem (Statement only) and
its simple applications (Scope as in Chapter 15, Sections 15.1 – 15.3 of Reference 1) (7
Lectures)
Conformal Mappings, Linear Fractional Transformations (Scope as in Chapter 12,
Sections 12.5, 12.9 of Reference 1). (8 Lectures)
References:
1. E. Kreyszig. Advanced Engineering Mathematics, Eighth Edition, John Wiley.
2. G. B. Thomas, R. L. Finney: Calculus, Ninth Edition, Pearson Education.
3. Michael D. Greenberg. Advanced Engineering Mathematics, Second Edition,
Pearson Education.
4. R. V. Churchill, J. W. Brown. Complex Variables and Applications, Sixth
Edition, McGraw-Hill, Singapore, 1996.
5. Vivek Sahai, Vikas Bist. Linear Algebra, Narosa Publishing House, New Delhi,
2002.
SYLLABUS
B.E. IN BIOTECHNOLOGY
FOURTH SEMESTER
Paper Title: Concepts in Bio-Technology-II (Theory)
Paper Code: BIO 401 Max. Marks:100 Credits: 4
Course Duration: 45 Lectures of one hour each.
Note for the Paper setter: The question paper should be divided into Section A and
Section B Total of 8 questions, 4 from section A and 4 from section B are to be set.. The
students will be required to attempt 5 questions selecting at least 2 from each section.
SECTION-A
1. Applications of Bio-Technology
a) Molecular diagnostics and therapeutics
b) Immunological diagnostic procedures
c) DNA diagnostics systems
d) Pharmaceutical diagnostics
e) Vaccines
2. Genetic Engineering of plants and animals
SECTION-B
3. Bioremediation and Biosensors
a) Clean up of oil spills
b) Clean up of soil contaminated with pesticides etc.
c) Heavy metal biosorption
d) Biodegradation of organic compounds
e) Biosensors in the field of biology
4) Regulations and Patenting in Bio-Technology
a) Biosafety levels of laboratories
b) State level regulations to be followed in Bio-Technology laboratories
c) Patents and biological products/processes
Books Recommended:
1. Setlow, J.K., (Ed): Genetic Engineering: Principles and Methods, 2006,
Springer. U.S.
2. Glick BR, Pasternak JJ: Molecular Biotechnology, ASM Press, 2003
3. Buterworth Heineman pub. :Techniques usedin Bioproducts Analysis-Biotol
Series (I-IV), author-BIOTOL BOARD,1992 ,UK
4. Ehebier :Lab. Techniques in Biochemistry and Molecular Biology,
New York.
5. Aastroel et al :Current Protocols in Molecular Biology, Green Publishing
Associates Inc and John Wiley & Sons, Inc
Paper Title: Concepts in Bio-Technology-II (Practical)
Paper Code: BIO 451 Max. Marks:50 Credits: 4
Practicals:
1. Isolation of DNA and its visualization through electrophoresis.
2. Southern blot hybridization
3. Collection and cultures of microbes from some polluted spot and see their
degradable activity.
4. Practice for filling up patent application.
Paper Title: Chemical Reaction Engineering(Theory)
Paper Code: BIO403 Max. Marks: 100 Credits: 4
Course Duration: 45 Lectures of one hour each
Note for the Paper setter: The question paper should be divided into Section A and
Section B Total of 8 questions, 4 from section A and 4 from section B are to be set.. The
students will be required to attempt 5 questions selecting at least 2 from each section.
SECTION-A
Introduction and brief review of the Kinetics of Homogeneous reactions (04)
Interpretation of rate data from Constant volume and Constant pressure systems. (08)
Single ideal reactors (04)
Design for single reactions (04)
Biochemical Kinetics: Interpretation of batch kinetic data; kinetics of enzymes catalyzed
reactions in free and immobilized states; Michaelis-Menton equation and its various
modifications; (04)
SECTION – B
Design for multiple reactions (08)
Biochemical Kinetics: Monod growth model and its various modifications; transport
phenomena in bioprocess systems; Effects of external mass transfer in immobilized
enzymes systems; analysis of intraparticle diffusion and reaction; kinetics of substrate
utilization , product formation and biomass production. (06)
Thermal Characteristics of Reactors: Temperature and pressure effects. (07)
Books Recommended:
Chemical Reaction Engineering :Levenspiel,D(John Wiley), 3rd edition,
1998.
Chemical Engineering or Chemical Kinetics : Smith, J.M.(Mc-Graw Hill) than 3rd
ed.,digitized in 2007.
Reaction Kinetics for Chemical Enginners: Wales, S.M.( Mc-Graw Hill), 3rd edition.
Chemical Reactor Theory - An introduction : Denbigh,K.Turner,K.K.(Cambridge Press)
3rd rev. edition., C U P Archive.
Elements of Chemical Reaction Enginnering : Scott Foggler, H.(PHI), 4th edition, 2006
Paper Title: Chemical Reaction Engineering(Practical)
Paper Code:BIO 453 Max. Marks: 50 Credits: 1
1. Kinetic studies in a batch reactor.
2. Kinetic studies in a plug flow reactor.
3. Kinetic studies in a CSTR.
4. Kinetic studies in a semi-batch reactor.
5. RTD studies in CSTR.
6. Dispersion number for packed bed reactor.
7. Adiabatic batch reactor.
Paper Title: Industrial Microbiology (Theory)
Paper Code: BIO 404 Max. Marks : 100 Credits: 4
Course Duration: 45 Lectures of one hour each.
Note for the Paper setter: The question paper should be divided into Section A and
Section B Total of 8 questions, 4 from section A and 4 from section B are to be set.. The
students will be required to attempt 5 questions selecting at least 2 from each section.
SECTION-A
1. Industrially important microbes (E. coli, Bacillus, Actinomyces, Saccharomyces ).
2. Preparation of an ideal growth medium for production of biomass and a microbial
product.
3. Strain improvement by genetic means. .
4. Culture preservation.
5. Classifications of microbia1 products.
6. Microbial fermentations, Introduction to design of fermentors.
SECTION-B
7. Microbial processes for: the production of organic acids, solvents, antibiotics
enzymes, . polysaccharides, lipids, pigments & aroma
8. Equipments and accessories for industrial processes.
9. Microbial enzymes, their stability. Enzyme stabilization by selection and
genetical engineering; protein engineering, reaction environment rebuilding,
chemical modification, intra-molecular cross linking and "immobilization, role of
enzymes" in bio-conversions of industrially important compounds.
10. Genetically engineered microbes and their regulation: Introduction, their
production, limitations and applications. .
Books Recommended:
1. M. J. Pelczar and E.C.S. (Jr) Chan, 2000 : Microbiology, Tata McGrawHil1
Pub.Co.;New"Delhi,5thed, 1986,digitize d on july-2008.
2. Davis.Bernard.D, R.Dulbecco, H.N.1990 Medical Microbiology, Harper and
Row, New York, 2nd edition.
3. M. J. Waites et al., 2001 : Industrial Microbiology, Blackwell Science Ltd.,
London, 3rd edition.
4. Nicklin et al., : Instant Notes in Microbiology, BIOS Scientific Publishers,
Ltd., U.K.
5. G.J.Tortora et al.1995:Microbiology: An Introduction. Benjamin/Cummings
Pub. Co., Inc, 10th ed., 2009.
Paper Title: Industrial Microbiology (Practical)
Paper Code: BIO 454 Max. Marks: 50 Credits: 1
Practicals :
1. Microbial cell growth kinetics.
2. Determination of size and density of the microbial cells; determination of thermal
death rate constant.
3. Estimation of efficiency of a glass-wool air-filter; preparation of immobilized
whole cell system,.
4. Production of ethanol, acids, solvents in microbial systems.
Paper Title: Immunology and Immunotechnology (Theory)
Paper code: BIO 405 Max. Marks: 100 Credits: 4
Course Duration: 45 Lectures of one hour each.
Note for the Paper setter: The question paper should be divided into Section A and
Section B Total of 8 questions, 4 from section A and 4 from section B are to be set.. The
students will be required to attempt 5 questions selecting at least 2 from each section.
SECTION-A
1. Introduction and historical perspectives of immune system. (2)
2. Cell and Tissues of immune system: Lymphoid cell, mononuclear cell,
granulocytes, mast cells, dendritic cells, primary lymphoid organs, lymphatic
system, secondary lymphoid organs.
(4)
3. Antigens and Haptens: Immunogenecity, chemical composition, susceptibility to
antigen processing, immunogen dosage and route of administration, haptens,
adjuvants. (4)
4. Antibody Structure, Function and Antibody Diversity: Basic structure,
Immunoglobulin domains, classes. (6)
5. Major Histocompatiblity Complex: MHC molecules, cellular distribution, general
importance. (3)
6. Antigen processing and Presentation to T cell: Antigen presenting cells and their
role, pathway.
(4)
SECTION-B
7. Antigen recognition and activation of immune response. (4)
8. Autoimmunity : Organ specific and systematic autoimmune diseases. (3)
9. Hypersensitive Reactions: Types, mechanisms of hypersensitivity. (3)
10. Complement System: Components, Complement activation, consequences. (4)
11. Antigen-antibody reactions, interaction, cross reactions, precipitation and
agglutination.
12. Immunoassays, radioimmunoassay, Enzyme linked immunosorbent assay,
Western blotting. (4)
13. Active and Passive immunization , polyclonal and monoclonal antibodies and
various types of vaccines. (4)
Books Recommended:
1. Goldsby, Kindt, T.J., and Osborne, B. A. 2000 : Kuby Immunology, W.H.
Freeman & Co., NY. 6th edition, 2007.
2. George Pinchuk, 2004 : Schaum’s Outline of Theory and Problems of
Immunology, Tata McGraw Hill Edition, 1st edition, 2001.
3. Abbas, A. K., Lichtman, A. H. & Pober, J. S., 2000: Cellular and
Mol.Immunology,. Philadelphia, 6th Edition., 2007, U.S.A, digitized on June
2008, Saunders Elsevier.
4. I.M. Riott, 2000 : Essential Immunology, ELBS/ Blackwell Scientific
Publications, U.K, 11th edition, 2006.
Paper Title: Immunology and Immunotechnology (Practical)
Paper Code: BIO 455 Max. Marks:50 Credits: 1
Practicals:
1. TLC and DLC for blood samples.
2. Determination of cell number (viable/non-viable).
3. Ficoll density gradient, separation of cell types.
4. Immunization methods: Preparation of polyclonal antibodies.
5. Determination of blood group antigens by hemeagglutination assay
6. Radial immunodiffusion Assay.
7. Ouchterlony Double Diffusion Assay.
8. Characterization of immunobiologicals by ELISA.
Paper Title: Legal Laws & IPR
Paper Code: BIO 406 Max Marks: 100 Credits: 4
Course Duration: 45 Lectures of one hour each.
Note for the Paper setter: The question paper should be divided into Section A and
Section B Total of 8 questions, 4 from section A and 4 from section B are to be set.. The
students will be required to attempt 5 questions selecting at least 2 from each section.
SECTION- A
Basics of Computer & Internet Technology:
Internet, ISP & domain name; Network Security; Encryption Techniques and Algorithms;
Digital
Signatures.
Introduction to Cyber World:
Introduction to Cyberspace and CyberLaw; Different Components of Cyber Laws; Cyber
Law and citizens.
E-Commerce :
Introduction to E-Commerce; Different E-Commerce Models; E-Commerce. Trends and
Prospects; E-Commerce and Taxation; Legal Aspects of E-Commerce.
SECTION-B
Intellectua1 Property Rights:
IPR Regime in the Digital Society; Copyright and Patents; Intenational Treaties and
Conventions; Business Software Patents; Domain Name Dispute and Resolution.
IT Act, 200l :
Aims and Objectives; Overview of the Act; Jurisdiction; Role of Certifying Authority;
Regulators under IT Act; Cyber Crime-offences and Contraventions; Grey Areas on IT
Act.
Project Work:
Candidates will be required to work on a project. At the end of the course students will
make a presentation and submit the project report.
Books Recommended:
1. Nandan Kamnath Laws Relating to computer, Internet, and E- Commerce(A
Guide to Cyber Laws & IT Act 2000 with Rules & Notification.), 2nd edition,
2000.
2. Keith Merill & Deepti Chopra Cyber Cops, Cyber Criminals & Internet
(IK Inter), 2002.
3. Diane Row Land Information Technology Law, 2005, 4th
edition2009.
4. Vakul Sharma Handbook of Cyber Laws (Mc.Mi1lian),2002.
SYLLABUS
B.E. IN BIOTECHNOLOGY
FIFTH SEMESTER
Paper Title: Enzyme Engineering & Technology (Theory)
Paper Code: BIO501 M. Marks: 100 Time: 3 hrs
Course Duration: 45 Lectures of one hour each.
Note for the Paper-setter: Total of 8 questions, 4 from section A and 4 from section B are
to be set.. The students will be required to attempt 5 questions selecting at least 2 from
each section.
Section - A
Introduction & Scope; General distinctive features and industrial applications; enzyme
kinetics; single, substrate steady state kinetics; King-Altman’s method; inhibitors and
activators; effect of pH and temperature; multi-substrate systems allosteric enzymes. (23)
Section - B
Immobilization of enzymes; advantages; carriers; adsorption; covalent coupling; cross
linking and entrapment methods; micro-environmental effect; enzyme reactors; reactors
for batch/continuous enzymatic processing, choice of reactor type; idealized enzyme
reactor systems; mass transfer in enzyme reactors; steady state analysis of mass transfer
and biochemical reaction in enzyme reactors bio-process design; physical parameters,
reactor operational stability; operational strategies; a few case studies. (22)
Paper Title: Enzyme Engineering & Technology (Practical)
Paper Code: BIO551 M. Marks: 50 Time: 2 hrs
Assay of enzymes; substrate specificity and efficiency of enzymatic catalysis;
Kinetics of enzyme catalyzed reactions,
Immobilization of enzymes,
Microenvironmental effects in immobilized enzymes.
Books Recommended
1.Biotechnology By W.F. Crueger & Crueger; 2003 (Text Book of Industrial
Microbiology), 2nd ed., 1991, digitized on Nov 2007.
2.Biochemical Engineering Fundmentals by J.E. Bailey and P.F. Ollis McGraw Hill
Publications, 2nd edition. 1986, digitized on Dec-2007,
3.Biotechnology Vol. 7 by H.J.R. Rehen and H.G. Reed; Beelar Publishers.
4.Bioprocess Engineering Basic Concepts, by P.H. M.L.Shuler , F.Kargi :1992,
Prentice Hall digitized on Dec-2007.
Paper Title : Bio-Process Engineering (Theory)
Paper Code : BIO 502 Max. Marks : 100 Time : 3 Hrs.
Course Duration : 45 lectures of one hour each.
Note for the Paper-setter: Total of 8 questions, 4 from section A and 4 from section B are
to be set.. The students will be required to attempt 5 questions selecting at least 2 from
each section.
Section - A
Kinetics of Microbial growth, substrate utilization and product formation in Batch, Fedbatch
and continuous processes. (10)
Rheology of fermentation fluids and Scale-up concepts. (05)
Introduction to modeling of growth kinetics: General structure for kinetic models,
overview of structure and unstructured models. (08)
Section - B
Sterilization of media: design of heat sterilization processes; kinetics; Sterilization in
place and Cleaning in place concepts. (06)
Sterilization of air : Filter sterilization and kinetics. (04)
Design of fermentation media and optimization. (06)
Aeration and agitation : various correlations and mass-transfer aspects, kLa
determination. (06)
Paper Title : Bio-Process Engineering (Practical)
Paper Code : BIO 552 Max. Marks : 50 Time : 2 Hrs.
Study of different phases of microbial growth;Estimation of cell mass; Growth rate;
mass and energy balance in a typical bioconversion process;
Concept of limiting nutrient and effect of its concentration on cell growth;
Study of growth inhibition kinetics;
Comparison between aerobic and anaerobic bioconversion processes;
Power consumption in a fermentation process and its correlation with rheology of the
fermentation fluid ;effect of speed on the mixing time in a bioreactor;
Estimation of kLa in a fermentation process.
Books Recommended:
1. Stainbury and whitakar : Principles of Fermentation Technology, Orgamon
Press.,2nd edition, 1995.
2. . M.L.Shuler , F.Kargi : Bioprocess Engineering Basic Concepts, 2nd edition,
2002, Prentice Hall.
3. Nielson J. Villadsen J. : Bioreaction Engineering Principles.
2nd Edition, 2003, Springer.
4. A.L. Demain; J.E. Davis : Manual of Indutrial Microbiology and
Biotechnology, 1999 ASM Press, Washington DC,
recented. 2010
5. M. Moo. Young : Comprehensive Biotechnology; The principles,
applications and regulations of Biotechnology in
Industry, agriculture and Medicine (4 Volumes)
Pergamon, Publishres. V-2, 1985, digitized on Dec-
2007.
Paper Title: Animal Cell Culture & Bio-Technology (Theory)
Paper Code: BIO503 M. Marks : 100 Time: 3 hrs
Course Duration: 45 Lectures of one hour each.
Note for the Paper-setter: Total of 8 questions, 4 from section A and 4 from section B are
to be set.. The students will be required to attempt 5 questions selecting at least 2 from
each section.
Section-A
Animal Cell metabolism, regulation and nutritional requirement; Animal cell growth
characteristics and kinetics; nutrients, substrate and product transport through mammalian
cell; primary & secondary culture; cell culture in continuous, perfusion and hollow-fiber
reactor; mass transfer in mammalian cell culture; scale-up of cell culture processes; case
studies.
Section-B
Gene transfer in animal cells and its applications; contamination & cyno presentation;
Transgenese and transgenic animals including live stock; Transgenics as bioreactors ;
Biotechnology or aquaculture, silkmoth, past control; Biodiversity, characterization,
conservation; In vitro fertilization, embryotransfer technology; Stem cell Biology &
Cloning.
Paper Title: Animal Cell Culture & Bio-Technology (Practical)
Paper Code: BIO553 M. Marks: 50 Time: 2 hrs
i. Preparation of cell culture medium.
ii. Establishment of Primary Culture;
iii. Establishment of culture of adherent cell line.
iv. Subculturing of non-adherent cell line
v. Cryopreservation of cultured cells.
vi. Revival of cryopreserved cell lines into culture.
Books Recommended:
1. Animal Biotechnology Vol 1 and II by Griffith and Spier, 6th edition, 1994,
Acadamic Pr.
2. Animal Cell Culture by Freshney, 3rd edition, 2009, digitized on Feb-2010.
Paper Title: Transport Phenomena (Theory)
Paper Code BIO 504 Max marks 100 Time 3 hours
Course Duration: 45 Lectures of one hour each.
Note for the Paper setter: The question paper should be divided into Section A and
Section B Total of 8 questions, 4 from section A and 4 from section B are to be set.. The
students will be required to attempt 5 questions selecting at least 2 from each section.
SECTION-A
Transport of momentum, heat and mass by molecular motion-Newton’s law of viscosity,
Fourier’s Law of heat conduction, Fick’s Law of Diffusion.
Transport properties-Viscosity, Thermal Conductivity and mass diffusivity.
Development of mathematical models of transfer processes through shell momentum
balance for solving specific problems of transport of momentum in laminar flow or in
solids in one dimension.
Development of general differential equations of fluid flow and their applications in
solving one-dimensional steady state and unsteady state problems of momentum transfer.
Emphasis on the analogy between momentum heat and mass transfer with respect to
transport mechanism and governing equations.
SECTION -B
Development of mathematical models of transfer processes through shell energy balance
and shell mass balance for solving specific problems of transport of heat and mass in
one dimension.
Development of general differential equations for heat transfer and mass transfer
and their applications in solving one-dimensional steady state and unsteady state
problems of heat and mass transfer.
Dimensional Analysis.
Books recommended:
Transport Phenomena Bird, R.B., Stewart, W.E.(John Wiley and Sons.),
2nd edition, 1962.
Fundamentals of momentum Weity,J.R., Wilson, R.E.,and
Wicks,C.E.(JohnWiley and
Heat and mass transfer sons), 4th edition.
Momentum Heat and Mass Bennett, C.O., Myers, J.E.(McGraw Hill), 3rd
edition.
Transfer
Paper Title: Transport Phenomena (Practical)
Paper Code: BIO 554 Max Marks 50 Time: 2 hrs
1. Thermal Conductivity of solids
2. Measurement of viscosity of liquids
3. Measurement of diffusivity of a binary system
4. Mass transfer coefficient for vaporization of naphthalene in air.
5. Heat transfer in natural convection.
6. Heat transfer in forced convection.
7. Wetted wall column: to find the mass transfer coefficient as a function of gas
mass velocity in a wetted wall column.
Paper Title: Bioprocess Technology
Paper Code: BIO505 M. Marks 100 Time: 3 Hours
Course Duration: 45 Lectures of one hour each.
Note for the Paper-setter: Total of 8 questions, 4 from section A and 4 from section B are
to be set.. The students will be required to attempt 5 questions selecting at least 2 from
each section.
Section – A
Introduction : Characteristics and comparison of bioprocess technology with chemical
technology. Substrates for bioconversion processes and design of media. Industrial
application of cells and enzymes, Process technology for the production of cell Biomass,
Ethanol, acetone-butanol, Citric acid, Dextran, aminoacids. (15)
Microbial production of some industrially important enzymes, Glucose isomerase,
cellulose. (05)
Section – B
Process technology for the production of penicillin, tetracycline, steroid. Process
technology for the production of vaccines, Bioenergy production; Bio-polymer design
and synthesis; Fermentation technology for waste stabilization and bio-product synthesis.
(24)
Books Recommended:
1. S.O. Enfors and L.H. Hagstrom, Bioprocess Technology- fundamentals and
applications, 1992. Cambridge University Press.
2. T.D. Brock Biotechnology : A Text of Industrial Microbiology, Smaeur
associate (1990).2nd ed., 1991, Sinaur Associates.
3. L.E. Casida Industrial Microbiology, wiley eastern Ltd. 1989,2nd ed.
4. M.Y. Young (Eds) Comprehensive Biotechnology, Vol. 1-4, Pergamon Press, 1985.
5. W. Crueger, A. Crueger: Bio-Technology, A Handbook of Industrial Microbiology,
2nd ed., 2000,Ponima pub.
6. Encyclopedia of Bioprocess Technology, Vol. 1-5, 1st ed.
Paper Title: Bioinstrumentation (Theory)
Paper Code: BIO506 M. Marks : 100 Time: 3 hrs
Course Duration: 45 Lectures of one hour each.
Note for the Paper-setter: Total of 8 questions, 4 from section A and 4 from section B are
to be set.. The students will be required to attempt 5 questions selecting at least 2 from
each section.
.
Section - A
Introduction: Problems unique to bioinstrumentation; Lab View: A graphical
programming language for virtual instrumentation;
Basic Principles: Review of system concepts input/output characteristics, the black box
signals linear, time-invariant systems static characteristics dynamic characteristics time
versus frequency domain analysis Fourier Analysis; Fourier transforms of common and
important signals windowing Discrete Fourier Transforms The Fast Fourier Transform
Spectrum Analyzers windows Frequency Analysis transfer functions, frequency response
magnitude and phase functions signals through systems 'ideal' and 'best' systems time vs
frequency domain filters how to measure frequency response in the laboratory; Sample
systems in the time and frequency domains; 0th, 1st, and 2nd order systems; Non-ideal
systems noise and signal-to-noise ratio; nonlinearities and distortion products.
wave-analyzer synchronous [lock-in] detection modulator, demodulator digital
techniques analog to digital conversion signal averaging V. Transducers and associated
electronics displacement transducers resistive strain gages bridge circuits capacitive
displacement transducers piezoelectric transducers optical transducers temperature
transducers;
Hemodynamic Measurements
Blood pressure, pressure transducers (manometers) extra-vascular techniques blood
sounds stethoscopes intra-vascular techniques catheter-manometers
Blood Volume and Flow indicator-dilution methods electromagnetic flow meters
ultrasonic methods.
Section - B
Bioelectric Potentials-Electrophysiology
Origins of biopotentials dipoles remote versus local potentials muscle and nerve cells,
intracellular potentials resting potential action potential stimulators discriminators
histograms remote potentials evoked potentials; example: evoked potential audiometry
Electrodes electrode interfaces fluid-fluid salt-fluid metal-fluid surface (skin) electrodes
metal micro-electrodes glass micro-electrodes microelectrode preamplifiers
Recording and Stimulating Systems, Putting it all together interference minimization and
rejection stimulus isolation shielding grounding ground-loops; Sample recording system:
electrocardiogram; ECG instrumentation
Other cardiac devices ; Pacemakers power and pulse sources electrodes; Defibrillators
Introduction to medical imaging
Books Recommended:
1. James Diefenderfer : Principles of electronics Instrumentation,
Publishers: Words worth for Black Box, 1989, 3rd
edition.
2. L.A. Geddes and Baker, L.E. : Principles of applied Biomedical Instrumentation,
A Wiley Interscience publication, 1989, 3rd edition.
3. Khandpur, R.S. : Handbook of Biomedical Instrumentation, Tata
McGraw Hill, 2nd Edition.
4. G. John : Medical Instrumentation application & Designs,
Webster, Publ., John Wiley & Sons, Inc., 2009.
5. J.R. Cameron : Homodynamic Measurements, Medical Physics.
6. J. Joseph Carr & M. John Brown : Introduction to Biomedical Equipment
Technology, Fourth Edition Pearson Education
Asia.
7. Cromwell et al. : Additional Reading: Biomedical Instrumentation
and measurements, Pub: prentice Hall of India, New
Delhi., 2nd edition.
BIO507 Training of 4 weeks after 4th semester exams: 50 Marks
SYLLABUS
B.E. IN BIOTECHNOLOGY
SIXTH SEMESTER
Paper Title: Recombinant DNA Technology (Theory)
Paper code: BIO601 Max. Marks:100 Time:3Hours
Course Duration: 45 Lectures.
Note for the Paper setter: Total of 8 questions may be set covering the whole syllabus.
Candidate will be required to attempt any 5 questions.
SECTION-A
1. Gene cloning and need to clone a gene; Isolation and purification of plasmid,
chromosomal and genomic DNA from bacterial, plant and animal cells. (10)
2. Different cloning vectors like plasmids, cosmids, phagemids, shuttle vectors,
and other vectors for plant and animals; enzymes used in recombinant DNA
technology like restriction endonucleases, ligases, polymerases, kinases
and phosphatases. (10)
SECTION-B
3. Cloning of a specific gene; studying gene location and structure; studying gene
expression; expression of foreign genes in research and biotechnology; maximization of
recombinant proteins; brief introduction to sequencing and site directed mutagenesis,
different types of PCR and applications; safety measures and regulations for recombinant
DNA work (15)
4. A brief introduction to the followings: phage display system, Yeast two hybrid system,
and RNAi technology. (5)
5. Applications of recombinant DNA technology in the fields of Medicine, Agriculture,
Forensic and Environment. (5)
Paper Title: Recombinant DNA Technology (Practical)
Paper code: BIO651 Max. Marks:50 Time:2Hours
Digestion of plasmid DNA by restriction endonuclease; Ligation assay; Amplification of
DNA using PCR; RAPD PCR; Induction and expression of a gene cloned in an
expression vector in E.coli..
Books Recommended :
1. Watson, J.D., et al. : Recombinant DNA: Genes and
(3rd ed.) Genomes,McGraw Hill Publications, 2007, H.
.W Freeman pub.
2. Brown, T.A. (4th ed.) : Gene Cloning and DNA analysis, An
Introduction,Blackwell Science.
3. Watson, J. (5th ed.) : Molecular Biology of the Gene, Benjamin,
Cummings, Pearson education, Schweiz AG,
Germany, 2004.
4. Primrose, S.B. and Twyman, R.M.: Principles of gene manipulation, Blackwell
and Old, R.W. (6th ed.) Science, U.K.
5. Alberts, B. et al . : Molecular Biology of Cell, Garland Publishers
Inc.,London,1994.
SIXTH SEMESTER B.E. (BIO-TECHNOLOGY) SYLLABUS
Paper Title: Operations Research
Paper Code: BIO602 Maximum Marks: 100 Time
of examination: 3hrs.
Course Duration: 45 lectures of one hour each.
Note for the paper setter: Total of 8 questions may be set covering the whole syllabus.
Candidate will be required to attempt any 5 questions selecting at least two from each
part.
Syllabus:
PART A
Optimization Problems. Linear Programming: Graphical Method (Scope as in
Chapter 1 of Reference 1).
Solution of simultaneous linear equations: An overview (Scope as in Chapter 2, Sections
2.15 – 2.16 of Reference 1).
Basic solutions, lines and hyperplanes, convex sets, extreme points, convex sets and
hyperplanes (Scope as in Chapter 2, Sections 2.19 – 2.21 of Reference 1).
Reduction of any feasible solution to a system of equations to a basic feasible solution.
Simplex Method: The simplex algorithm (Scope as in Chapter 3, 4 of Reference 1).
Tableau format for simplex computations, Charne’s M-method, Two phase method
(Scope as in Chapter 5 of Reference 1).
The revised simplex method (Scope as in Chapter 7 of Reference 1). (12 Lectures)
Duality theory: Formulation of the dual problem, Theorems on duality: Weak Duality
Theorem, Strong Duality Theorem, Complementary Slackness Theorem, Dual Simplex
Algorithm (Scope as in Chapter 8, Sections 8.1 – 8.12 of Reference 1). (6 Lectures)
Integer Linear Programming: Branch and Bound Algorithm, Cutting Plane Algorithm
(Scope as in Chapter 9, Section 9.1 – 9.2 of Reference 2). (4 Lectures)
PART B
Transportation Problem: Initial solution by North-West corner rule, Row minima
method, Column minima method, Matrix minima method, Vogel’s method. Tableau of
transportation problem, u-v algorithm for solving transportation problem. Degeneracy in
transportation problem. (Scope as in Chapter 9 of Reference 1). (6 Lectures)
The Assignment Problem: Hungarian Method (Scope as in Chapter 5, Section 5.4 of
Reference 2). (2 Lectures)
Traveling Salesman Problem (Scope as in Chapter 9, Section 9.3 of Reference 2).
(2 Lectures)
Dynamic Programming: Shortest route problem, Knapsack Model, Workforce size
model, Equipment replacement model, Investment model, Game of chance (Scope as in
Chapter 10, Sections 10.1 – 10.3, Chapter 15, Section 15.1 – 15.2 of Reference 2).
(6 Lectures)
CPM and PERT: Network representation, Critical path computations, Construction of
time schedule, Linear programming formulation of CPM, PERT networks (Scope as in
Chapter 6, Section 6.6 of Reference 2). (2 Lectures)
Basic Queuing Systems: Elements of a queuing model, Pure birth and pure death model,
Generalized Poisson queuing model (Scope as in Chapter 17, Section 17.1 to 17.5 of
Reference 2). (5 Lectures)
References:
1. G. Hadley. Linear Programming, Narosa Publishing House, New Delhi, 2002.
2. Hamdy A. Taha. Operations Research, An Introduction, Seventh Edition, Pearson
Education, Delhi, 2003.
3. Kanti Swaroop, P. K. Gupta, Man Mohan. Operations Research, Twelfth Edition,
Sultan Chand and Sons, New Delhi, 2004.
4. A. M. Natarajan, P. Balasubramani, A. Tamilarasi. Operations Research, Pearson
Education, Delhi, 2005.
Paper Title: Operation Research (Practical)
Paper Code: BIO652 Max. Marks 50 Time: 2 Hours
Practicals based on theory paper code BIO602
Paper Title: Introduction to Bioinformatics (Theory)
Paper Code: BIO 603 M. Marks : 100 Time: 3 hrs
Course Duration: 45 Lectures of one hour each.
Note for the Paper-setter: Total of 8 questions, 4 from section A and 4 from section B are
to be set.. The students will be required to attempt 5 questions selecting at least 2 from
each section.
Section –A
Introduction to Bioinformatics:
History of Bioinformatics; Objectives and areas of Bioinformatics; Genome sequencing
projects; Human Genome Project - history, techniques and insights.
Introduction to databases:
Basic concept of database, Type of databases;
Literature Databases-PUBMED, MEDLINE;
Nucleic acid and protein databases- GenBank, EMBL, DDBJ, SWISS PROT, UNIPROT;
Human, animal and plant databases- Ensembl, Genome project TIGR database, Maize
GDB etc.Structural databases- PDB, PDBsum, NDB etc;Motifs and Pattern Databases-
PROSITE, Pfam, BLOCKS, PRINTS etc;Database Retrieval and deposition systems-
SRS, Entrez, Bankit, Seqin, Webin, AutoDep.
Basic Sequence Analysis:
Protein Sequence Analysis- composition, hydropathy, flexibility, pattern, motif etc;
Nucleic acid Sequence Analysis- Composition, motif, restriction site, primer design etc.
Sequence Homology:
Scoring matrices, Local and global alignment concepts, Dot matrix sequence comparison,
Dynamic programming; Statistics of alignment score;
Database searches for homologous sequences- FASTA, BLAST, PSI-BLAST and PHIBLAST;
Multiple sequence alignment: CLUSTALW, PILEUP;
Evolutionary analysis- Concept of phylogeny and trees, Relationship of phylogenetic
analysis to sequence alignment.
Gene prediction:
Gene prediction in prokaryotic and eukaryotes genomes; evaluation of gene prediction
methods.
Section - B
Protein structure prediction:
Prediction of protein secondary structure from the amino acid sequence- Chou-
Fasman/GOR method, JPRED, PSIPRED, PHD;
Prediction of three-dimensional protein structure-Homology-based structure prediction,
Fold recognition and ab initio methods for structure prediction;
Evaluating the success of structure predictions-CASP and CAFASP.
Introduction to the concepts of molecular modeling:
Molecular structure and internal energy; Molecular Mechanics; Energy Minimization and
related methods for exploring the energy surface; Molecular Dynamics, Conformational
analysis;
Use of molecular graphics packages- Rasmol, MOLMOL, Chimera, Pymol, spdbviewer.
Computer Aided drug design:
Drug discovery process; Role of molecular recognition in drug design; Concepts in
Quantitative structure activity relationships (QSAR); Docking problem, Concepts of
docking; Structure based Drug design.
Applications of Bioinformatics:
Comparative Genomics; Proteomics; Gene expression informatics; Metabolomics;
Computer aided vaccine design.
Text / Reference Books:
1. Mount D. W. (2004). Bioinformatics & Genome Analysis. Cold Spring Harbor
Laboratory Press.
2. Baxevanis B.F. and Quellette F. (2004). Bioinformatics a Practical Guide to the
Analysis of Genes and Proteins. Wiley-Interscience.
3. Eidhammer I., Jonassen I. and Taylor W. R. (2004). Protein Bioinformatics: An
algorithmic approach to sequence and structure analysis. Mathematics.
4. Orengo C.A., Jones D.T. and Thornton J.M. (2003). Bioinformatics: Genes
Proteins and Computers. Bios Scientific Pub.
5. Bourhe P. E. and Weissig H. (2003). Structural Bioinformatics (Methods of
structural Analysis). Wiley-Liss.
6. C. Braden and C. Tooze (1991). Introduction to Protein Structure” Garland
Publishing Inc., New York.
7. Jiawei Han, Micheline Kamber, Data Mining Concepts and Techniques- Morgan
Kaufmann publisher, 2001.
8. S.C.Rastogi, N.Mendiratla and P.Rastogi (2004)., Bioinformatics methods and
applications- Genomics, Proteomics and Drug Discovery, Prentice Hall (India).,
9. T.B.Kitano (2003), Handbook of Comparative Genomics: Principles and
Methodology, Graziano Pesole.
10. Dov Stekal (2003) Microarray Bioinformatics, Cambridge University Press,
Cambridge.
11. Brown TA. (2002) Genomes. John Wiley & Sons (Asia) Pvt. Ltd. Singapore.
12. Charles R. Cantor, Cassandra L. Smith (1999) Genomics: The Science and
Technology behind the Human Genome Project, John Wiley & Sons (Asia) Pvt.
Ltd. Singapore.
13. P.Clate & R.Backofen (1998), Computational Molecular Biology, Willy
Publication,
14. T.K.Atwood and D.J. Parry Smith, Introduction to Bioinformatics. Biological
databases: chapters 3 and 4
Paper Title: Introduction to Bioinformatics (Practical)
Paper Code: BIO 653 Max Marks 50 Time: 2 hrs
a) To Study & analyse various biological databases at NCBI, EBI, Expasy, NBRFPIR
Nucleic acid sequence databases like Gene Bank, EMBL etc.
Protein sequence databases SWISSPROT, UNIPROT etc.
Structural databases- PDB, NBD
b) To retrieve sequences from NCBI/EBI/ExPasy using ENTRZ, SRS
c) Similarity searches using various tools like
BLAST/ FASTA, BLAST N, BLAST P, BLAST X
d) CLUSTALW / Phylogenetic analysis tools
e) To predict gene/ORF for genomic DNA sequences of prokaryotic and eukaryotic
origin.
f) To analyze protein sequence using Secondary Structure prediction Methods:
Chou-Fasman/GOR method, JPRED, PSIPRED, PHD etc.
g) Energy minimization using SPDBV.
h) To down-load structures of proteins in software like RASMOL, SPDBV and
analysis of structures in these software
i) Fold recognition
j) Homology modeling using SPDBV.
Paper Title: Bio-Reactor Design and Operation (Theory)
Paper Code: BIO 604 Max. Marks: 100 Time: 3 Hrs.
Course Duration: 45 lectures of one hour each.
Note for the Paper-setter: Total of 8 questions, 4 from section A and 4 from section B are
to be set.. The students will be required to attempt 5 questions selecting at least 2 from
each section.
.
Section - A
Introduction to reactor design and Kinetics of Bioreactions (review). (1)
Biological systems: Organism selection; bacterial, yeast and fungal cultures; Effect of
microorganism type and culture characteristics on bioreactor design and operation. (6)
Ideal Reactors: Batch reactor (Closed and fed-batch), continuous reactors; PFTR, CSTR
design equations. Reactors for biomass growth; reactors in series; recycle reactors;
overview of pneumatically agitated bioreactors; Membrane bioreactors and Photo
bioreactors. (10)
RTD in reactors: Models for non-ideal reactors; Tanks in series and dispersion models.
(6)
Section - B
Instrumentation and control of various parameters in bioreactors for Dissolved oxygen,
foam,pH, temperature, flow, pressure, microbial biomass, CO2 etc; Methods of
measuring process variables and control systems: Proportional, Proportional integral and
Proportional integral derivative. (8)
Operation and scale-up of bioreactors. (4)
Bioreactor modeling and stability: Fermentation dynamics, Biomass production and
dilution factor, Thermal stability concepts. (6)
Mechanical design Concepts: Application to continuous sterilizers, RTD concepts,
Application of design principles. (4)
Books Recommended:
1. M.L.Shuler , F.Kargi : Bioprocess Engineering Basic Concepts, P.H,
2nd edition, Prentice Hall.
2. J.A.Asenjo and J.C.Merchule : Bioreactor Sytem Design, dekker. C R C Pr. 1st
edition, 1994. (Eds.)
3. KK. Van’t R et and J. Tramper : Basic Bioreactor Design, marcel
Decker, 1991 C R C Pr.
4. J.M.Lee : Biochemical Engineering, PH 1992.
5. B. Atkinson : Biochemical Engineering and
Biotechnology, 2nd revised edition. Handbook,
Stockton Press, N.Y. 1992
Paper Title : Downstream Processing
Paper Code: BIO605 M. Marks 100 Time: 3 Hours
Course Duration: 45 Lectures of one hour each.
Note for the Paper-setter: Total of 8 questions, 4 from section A and 4 from section B are
to be set.. The students will be required to attempt 5 questions selecting at least 2 from
each section.
. Section – A
Introduction; An overview of Bioseparation, Separation of cells and other insolubles from
fermented broth; Filtration and microfilteration; Centrifugation (batch, continuous).
Designing of centrifuges for desired product of desired capacity; Cell disruption: Physical
methods – osmotic shock, grinding with abrasives solid shear, liquid shear, Chemical
methods- alkali reagents, enzymatic methods; Product isolation: Extraction and
adsorption method, solid-liquid separation, liquid-liquid separation, distillation,
precipitation method using ammonium sulfate, organic solvents, high molecular weight
polymers, reverse osmosis;
Section – B
Electrophoresis and chromatography principles for product purification. Different
electophoresis techniques viz. isoelectric focusing, chromatographic techniques viz.
paper, gel filtration, column, ion exchange, affinity, GLC, HPLC. Dialysis,
ulterafilteration; Product polishing: crystallization and drying.
Paper Title: Down Stream Processing (Practical)
Paper Code: BIO655 Max. Marks 50 Time: 2 Hours
Cell lysis and release of cell contents; Use of centrifugation, ultra centrifugation Ultra
filtration, lypophilization, crystallization, HPLC for biosparation.
Books Recommended:
Bailly & Ollis Biochemical Engg. – Academic Press, 2nd ed., TMH.
2.H.Gunzler – Handbook of Analytical techniques – Wiley Publications, 1st ed. , 2001.
3. H.J.Rehm and G.Reed, Biotechnology – Vol. 3, 4, 5 verlar Publishers, 2nd ed.,Wiley
VCH
4. Humphrey, Aiba and Miller, Biochemical Engg. Academic Press
5. Murray Moo-Young Comprehensive biotechnology, Vol. II latest ed. Pergan
Publishers, V-4,1985.
6. Wilson and Golding, A Biologist’s Guide to Principles & Techniques of Practical
7. Stanbury & Whitteker, Principles of Fermentation Technology, Pergamon Press, 1999,
2nd edition.
Paper Title : Biomaterials
Paper Code: BIO606 M. Marks 100 Time: 3 Hours
Course Duration: 45 Lectures of one hour each.
Note for the Paper-setter: Total of 8 questions, 4 from section A and 4 from section B are
to be set.. The students will be required to attempt 5 questions selecting at least 2 from
each section.
. Section - A
Introduction to biomaterials, Characterization of materials; mechanical properties;
thermal properties; surface properties and adhesion; Various classes and forms of
biomaterials;
Metals : Stainless steel, Cobalt- Chromium Alloys, Titanium based alloys other metals,
metallic corrosion and luological tolerance.
Cermics : Carbons alumina, recoverable ceramics, composites, ceramic surface analysis
Synthetic polymers : Polymers in luomedial use, polyethylene, polypropylene,
perfluorinated polymers. Acrylic polymers, hydrogels, polyurethanes, polyamide, silicone
rubbers, polymer sterilization.
Biopolymers : Collagen, Elastin, Mucopolysarrharides, Proteoglycans Cellulose and
derivatives and other.
Tissue grafts : Blood, Tissue grafts and rejection processes, shim and grafts
Soft tissue speciation : Space filters maxillofacial and fluid transfer implants, biomaterials in
urological practice.
Section - B
Cardiovascular implants and extra-coronial devices : Blood clotting, Blood Theology, Blood
Vessels, The Heart, Lungs and Vascular implants, Blood substitutes.
Biomaterials in ophthalmology : Anatomy of eye, Viscoelastic Solution Contact Lens
and Optical implants, Skeletal looking material for retinal detachment Vitreous implants
artificial tears.
Orthopedic implants : Bone composition and materials, fixation devices, Fracline
healing by clinical and dedromagnetic stimulation hip joint replacement, Knee joint
repair, bone regeneration with restorable materials.
Dental Materials : Tooth composition and mechanical properties impression materials,
filling and restorative materials, metal in dentist, oral implants use of collagen in dentist
Books Recommended :
1. Sujata V. Bhat : Biomaterials (2nd Edition), 2002, Springer.
2.Finman : Biomaterials
3. Ratner : Biomaterial and science, 2nd edition. Technomic.
B.E. (Biotechnology) Fourth Year-Seventh Semester (Syllabus)
BIO701 Environmental Biotechnology
Total Number of lectures: 45 M. marks: 100 Time: 3 hrs
Note for the Paper setter: The question paper should be divided into Section A
and Section B Total of 8 questions, 4 from section A and 4 from section B are to be
set.. The students will be required to attempt 5 questions selecting at least 2 from
each section.
SECTION-A
Introduction: Environmental Pollution, Sources and effects. Biodegradation and
Bioremediation-definitions and examples. (5)
Waste Water Treatment: Aerobic and Anaerobic waste water treatment. Kinetics for
waste water treatment processes. Different type and design of Aerobic and Anaerobic
reactors, Methanogenesis. Emerging biotechnological processes in waste water treatment.
(18)
SECTION-B
Solid Waste management: Biodegradation of pollutants, Treatment processes for solid
waste, Use and Management, Bioremediation of contaminant lands. Hazardous wastes:
Source management and safety. (10)
Microbial Leaching and Mining: Recovery of metals from solutions, microbes in
petroleum extraction, microbial desulphurization of coal. (7)
Environmental Genetics: Degradative plasmids, release of genetically engineered
microbes in environment. (5)
Practical:
To determine BOD & COD levels of the sample. Application of microbial approaches
in the remediation of contaminated soils. Analysis of contaminated and bioremediated
samples.
Reference Books
1. Bruce E. Rittmann & Perry L. McCarty. Environmental Biotechnology: Principles
& Applications (1st Edition), McGraw-Hill, 2000.
2. Michael T. Madigan, John Martinko & Jack Parker. Brock Biology of
Microorganisms (10th Edition). Prentice Hall, 2002.
3. George Tchobanoglous, Franklin L. Burton Editor & H. David Stensel
Wastewater Engineering: Treatment and Reuse (4th Edition). McGraw-Hill, 2002.
4. Comprehensive Biotechnology (Vol. 1-4): M. Y. Young (Eds.), Pergamon Press,
Oxford.
Paper Code : BIO 702 Food Biotechnology
Course duration: 45 Lectures of one hour each. M.Marks: 100 Time: 3 hrs
Note: The question paper should be divided into Section A and Section B Total of 8
questions, 4 from section A and 4 from section B are to be set.. The students will be
required to attempt 5 questions selecting at least 2 from each section
SECTION-A
History of Microorganisms in foods (2)
Primary sources of Microorganisms in foods (3)
Synopsis of common food borne bacteria, fungi and yeasts (6)
Incidence & Behavior of Microorganisms in foods (3)
Extrinsic & Intrinsic parameters of foods (4)
Role and Significance of Microorganisms as Single cell proteins, Food value of
Mushrooms, Yeasts, Production of Fermented foods (3)
SECTION-B
Food types and their physical & chemical properties (4)
Food Spoilage (3)
Food Borne diseases (6)
Food Preservation (5)
Diagnosis of microbial contents of food: Classical & Molecular approach (4)
Food Biosensors (2)
Practical:
Microbiological Examination of food/s. Enumeration and detection of food borne
organisms. Estimation of quality of milk-by dye reduction, direct microscopic count,
Determination of diacetyl, titrable acidity in the milk sample.
Reference books
1. Morten Meilgaard, Gail Vance Civille & B. Thomas Carr. Sensory Evaluation
Techniques (3rd Edition). CRC Press, 1999.
2. Daniel Charles. Lords of the Harvest: Biotech, Big Money, and the Future of
Food (1st Edition). Perseus Books Group, 2001.
3. Adams, M.R. and M.O. Moss. Food Microbiology. Turpin Distribution Service
Ltd., Blackhorse Road, Letchworth, Herts SG6 1HN, UK, 1995, 2nd edition.
4. Gauri Mittal.Food Biotechnology: Techniques and Applications. CRC Press,
1992.
5. Banwart, George J. Basic Food Microbiology, 2nd ed. AVI/ Van Nostrand
Reinhold Publishing Co, 1989.
6. Cliver, D.D. Foodborne Diseases. Academic Press, Inc, 1990.
7. Food and Drug Administration. Bacteriological Analytical Manual (BAM) (8th
Edition). AOAC, Arlington, VA, 1995.
BIO703 Plant Tissue Culture
Total Number of lectures: 45 M. Marks: 100 Time: 3hrs
Note for the Paper setter: The question paper should be divided into Section A
and Section B Total of 8 questions, 4 from section A and 4 from section B are to be
set.. The students will be required to attempt 5 questions selecting at least 2 from
each section.
Section A
Introduction, requirements and Techniques. (4)
Tissue Culture Media and Cell Culturing. (5)
Cellular Totipotency, Somatic Embryogenesis, Synthetic seeds.
(6)
Haploid Production: Zygotic Embryo Culture: Morphogenesis in the culture of seeds
with partially differentiated embryos. (5)
Section B
Microsurgical experiments, Morphogenic potential of the embryo callus, practical
applications. (5)
In-Vitro Pollination and Fertilization: Introduction, Terminology, In-Vitro
Pollination, Applications. (5)
Genetic engineering and production of pathogen free plants: Gene expression, genetic
stability, and field performance. (5)
Introduction, Strategies used to optimize product yield, commercial aspects,
Germplasm storage: Introduction, long term Storage, Short or Medium term storage.
(10)
Reference Books:
1. Adrian Slater, Nigel W. Scott & Mark R. Fowler. Plant Biotechnology: The
Genetic Manipulation of Plants (1st Edition). Oxford University Press, 2003.
2. Roberta H. Smith. Plant Tissue Culture: Techniques and Experiments (2 Edition).
Academic Press, 2000.
3. Lydiane Kyte, John Kleyn & John G. Kleyn. Plants from Test Tubes: An
Introduction to Micropropagation (3rd Edition). Timber Press, 1996.
4. J. Heslop-Harrison, John H. Dodds & Lorin W. Roberts. Experiments in Plant
Tissue Culture (3rd Edition), Cambridge University Press, 1995.
BIO 704 Bioanalytical Techniques
Max. Marks: 100 Time: 3 Hours
Course Duration: 45 lectures of one hour each.
Note for the paper setter: The question paper should be divided into section A and
section B. Total of 8 questions, 4 from section A and 4 from section B are to be set. The
students will be required to attempt 5 questions selecting at least 2 from each section.
Section-A
Spectrophotometry(UV and Visible): Principle, single beam and double beam
spectrophotometer, factors influencing the absorption spectra, overview of empirical
rules, solvent perturbation method and difference spectroscopy; various applications of
absorption spectroscopy w.r.t. biotechnology.(05)
Spectrofluorimetry: Principle, significance and various details related to
instrumentation. (03)
Atomic absorption Spectrophotometry: Principle, instrumentation details, various
interferences in atomic absorption spectroscopy and applications.(04)
Infrared and Raman Spectroscopy: Principle, factors deciding the spectra,
instrumentation, overview of different class of compounds and their IR spectra.
Introduction to Raman scattering.(04)
Nuclear Magnetic resonance: phenomena of resonance, instrumentation, diamagnetic
shielding, anisotropy, chemical shift, free induction decay (FID), population distribution
of nuclei, and prediction of NMR spectra on the basis of (n+ 1) rule for basic class of
compounds. Overview of electron spin resonance spectroscopy (ESR) and magnetic
resonance imaging (MRI).(08)
Section-B
Electron Microscopy: Transmission and scanning electron microscopy, significance of
vacuum, basic instrumentation for TEM and SEM, sample preparation for electron
microscopy. Overview of Atomic force microscopy and tunneling microscopy w.r.t. their
working principle and comparison with other scanning techniques. (05)
Crystallography and X-ray diffraction: Introduction to x-ray and general theory and
instrumentation, Bragg’s law, various techniques to determine crystal structure.(05)
Radioisotope techniques: radiotracers, units of radioactivity measurement, proportional
and scintillation counters, introduction to autoradiography and nuclear medicine.(06)
Mass Spectroscopic techniques: Introduction to mass-spectroscopy, significance,
instrumentation details of a mass-spectrometer, ionization techniques, single and double
focusing, alternate mass separation techniques- time of flight and quadruple. Interface of
mass-spectra with liquid and gas chromatography (LC-MS and GC-MS)(05)
Practical:
Different experiments to confirm spectroscopic laws, practical demonstration of the
above mentioned techniques.
Suggested Books:
1. Keith Wilson & John Walker : Principles and Techniques of Practical
Biochemistry (5th Edition), Cambridge
University Press,2000.
2. William Kemp : Organic spectroscopy. English Language
Book Society & The Macmillan Press Ltd,
3rd edition.
3. David Friefelder : Physical Biochemistry, Freeman & Co.
New York, 2nd edition.
4. P. Carmona, R. Navarro,A. : Spectroscopy of Biological Molecules:
Modern trends( Ist Editon), Kluwer
Academic Publishers, 1997.
BIO 705: Minor Project
BIO706: TRAINING OF 4 WEEKS AFTER 6th SEMESTER EXAMS.
SYLLABUS FOR B.E. (BIO- TECHNOLOGY)
EIGTHTH SEMESTER
BIO 801 Major Project
BIO 802 Enzyme Catalyzed Organic Synthesis
Total Number of lectures: 45 Maximum marks: 100
Note for the Paper setter: The question paper should be divided into Section A
and Section B Total of 8 questions, 4 from section A and 4 from section B are to be
set.. The students will be required to attempt 5 questions selecting at least 2 from
each section.
SECTION-A
Biocatalysis: Definition of Biocatalysis, advantages and disadvantages of Biocatalysis
over chemical catalysis. Different types of Biocatalysis; microbial ,enzymatic and
enzymatic and immobilized system of Biocatalysis; current industrial biocatalysis with
different enzymes.
Immobilized enzymes for Biocatalysis. (15)
Industrial processes: Comparing different approaches. (8)
SECTION-B
Stereo selective biocatalysts for the synthesis of chiral pharmaceutical intermediate such
as synthesis of ACE inhibitors ,definition, mode of action of inhibitors ,recent
developments synthesis of anticholesterol drug by biocatalysis routs , calcium channel
blocking drugs, potassium channel openers, antiviral. (22)
Practical:
Optimization of enzymatic catalysis reaction conditions.
Enzymatic catalysis reaction with activators and inhibitors.
Product identification using different analytical technique.
Reference Books
1. Andreas S. Bommarius, Bettina R. Riebel. Biocatalysis: Fundamentals &
Applications, Wiley VCH, 2004.
2. Lawrence P. Wackett and C. Douglas Hershberger Biocatalysis and
Biodegradation: Microbial Transformation of Organic Compounds. ASM
Press, Washington DC, 2001.
3. Stanley M. Roberts, Nicholas J. Turner, Andrew J. Willets, Michael K. Turner.
Introduction to Biocatalysis: Using Enzymes and Microrgansims, Cambridge
University Press, 1995.
4. Sheldon, R.A., Porskamp, P., and Ten Hoeve, W. Advantages and Limitations of
Chemical Optical Resolution, in Biocatalysis in Organic Synthesis, Tramper, J.,
Van der Plas, H.C., and Linko, P. (Eds.) Elsevier, Amsterdam, 1985.
BIO 803 Project Management and Entrepreneurship
Total Number of lectures: 45 Maximum marks: 100
Note for the paper setter: The question paper should be divided into section A and
section B. Total of 8 questions, 4 from section A and 4 from section B are to be set. The
students will be required to attempt 5 questions selecting at least 2 from each section.
Objective: The course aims at acquitting the students with the subject of project
management and also nature, significance and problems of entrepreneur with special
reference to India.
SECTION-A
Project Formulations and Planning ,Private commercial criteria for project choice, project
cycle, feasibility, marketing feasibility, Financing for Projects and financial feasibility,
Project Implementation. Brief outline of social cost benefit analysis: rationale, UNIDO
and little Mirrlees approaches, UNIDO-IDCAS manual, shadow prices and conversion
factors, applications in India. Planning and scheduling networks, critical path, PERT
model, CPM model, PERT/cost, resource leveling and allocation.
SECTION-B
Entrepreneur- Concept on percent - Functions and clarifications of entrepreneurs -
Characteristics of entrepreneur - Nature and importance of ,entrepreneur - Entrepreneur
vs. professional manager - Women entrepreneurs. Concept of Entrepreneurship -
Entrepreneurship and environment-Policies governing entrepreneurs, entrepreneurial
development programmes - Institutions for - entrepreneurship development,
entrepreneurship. Entrepreneurship -Entrepreneurship development in other countries.
Institutions for Entrepreneurial Development - Role of constancy organizations - Role of
financial institutions -Bank finance to entrepreneurs Entrepreneurship development: Role
of development financial institutions.
Books Recommended
1. UNIDO: Guidelines for Project Evaluation, United Nations, reprinted,1993..
2. Mannual for the preparation of Industrial Feasibility Studies, United Nations
1995.
3. Manual for Evaluation of Industrial Projects, United Nations, reprinted on 1993..
4. IMD little and J.A. Mirrlees: Project Apraisal and Planning in Developing
Countries, 1975.
5. Prasanna Chandra: Projects: Preparation, Appraisal Budgeting and Control, 7th
edition, TMH.
6. Vasanta Desai: Dynamics of entrepreneurial development and management, 11th
edition, Himalaya pub.
7. Vasanta Desai: Entrepreneurial development, and Management, 13th edition,
Himalaya pub.,Harper Collins, edition- Paperback.
8. Peter F. Drucker: Innovation and development.
BIO 804 Modeling and Simulation of Bioprocesses
Total Number of lectures: 45 Maximum marks: 100
Note for the Paper setter: The question paper should be divided into Section A and
Section B Total of 8 questions, 4 from section A and 4 from section B are to be set..
The students will be required to attempt 5 questions selecting at least 2 from each
section.
SECTION-A
Types of kinetic models. Data smoothing and analysis. (8)
Mathematical representation of bioprocess; parameter estimation; numerical
integration techniques; parameter sensitivity analysis; statistical validity. (10)
Discrimination between two models. Physiological state markers and its use in the
formulation of a structured model. (5)
SECTION-B
Development of compartment and metabolic pathway models for intracellular state
estimation. (10)
Dynamic simulation of batch, fed-batch steady and transient culture metabolism;
Numerical optimization of Bioprocesses using Mathematical models. (12)
Practical:
To design mathematical models from the provided data.
Reference Books
1. John Ingham, Irving J. Dunn, Elmar Heinzle & J.E. Prenosil. Chemical
Engineering Dynamics: Modelling with PC Simulation (2nd Edition). Wiley-
VCH, 2000.
2. Jonathan B. Snape, Irving J. Dunn, John Ingham & J.E. Prenosil. Dynamics of
Environmental bioprocesses: Modelling and Simulation. Wiley-VCH, 1995.
3. Dieter Behrens & P. Kramer (Eds.). Bioreactors, Downstream Processing,
Process and Reactor Modelling and Bioprocesses. Wiley-VCH, 1988.
4. Dunn. Biological Reaction Engineering. John Wiley & Sons Inc
ELECTIVE - 1
BIO805 Nanobiotechnology
Total number of lectures: 45 Maximum Marks: 100
The question paper should be divided into section A and section B. Total of 8 questions, 4
from section A and 4 from section B are to be set. The students will be required to
attempt 5 questions selecting at least 2 from each section
SECTION A
Cell nanostructure interactions: Surface patterning to control the adhesion of cells
using
nanoscale topography and chemical modifications
Magnetosomes: Magnetotactic bacteria, Magnetite crystals, Magneto-aerotaxis,
Applications
Nanotherapeutics: Nanoshells, Dendrimers , Nanoparticles
Tissue engineering: Significance, Methodology, Tissue engineering scaffoldscomposition,
properties, fabrication, cell seeding and proliferation
Nanomembranes: Freely suspended nanomembranes
SECTION B
Bionanodevices: Nanosensors and Actuators
Nanopumps: Fabrication using SOI wafers
Nonorobots: Developing nanorobots for biomedical application
Molecular motors: Surface patterning and the control of motility of the actin/myosin
motor system
Engineered nanopores: Classes of nanopores, engineering techniques, potential
applications of nanopores
Reference Books
1. David S. Goodsell. Bionanotechnology: Lessons from Nature. (1st Edition) Wiley-
Liss, 2004.
2. Bharat Bhushan. Handbook of Nanotechnology (1st Edition). Springer Verlag,
2004.
3. Christof M. Niemeyer & Chad A. Mirkin (Eds.). Nanobiotechnology: Concepts,
Applications and Perspectives, 2004.
ELECTIVE – 2
BIO 805 Microbial Biodiversity
Total Number of lectures: 45 Maximum marks: 100
Note for the Paper setter: The question paper should be divided into Section A and
Section B Total of 8 questions, 4 from section A and 4 from section B are to be set..
The students will be required to attempt 5 questions selecting at least 2 from each
section.
SECTION A
Microbial biodiversity: definition and introduction, evolution and diversity of
microorganisms. (7)
Physiological and metabolic diversity of microorganisms. (7)
Microbial diversity of major ecosystems. (8)
SECTION B
Biodiversity & role of microorganisms in plants and animal symbiosis. (8)
Microbial diversity in extreme environments. (9)
Microbial biodiversity, biotechnology and future biodiversity. (6)
Reference Books
1. Jo Handelsman. Biology Brought to Life (Student Version) McGraw-Hill, 2002.
2. Oladele Ogunseitan. Microbial Diversity: Form and function in Prokaryotes.
Blackwell Publishers, 2004.
3. 3. Alan T. Bull (Editor). Microbial Diversity and Bioprospecting. American
Society Microbiology, 2003.

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