Saturday, 3 March 2012

Cell biology , Semester I, MSc. BIOTECHNOLOGY CURRICULUM- 2009-2011


Unit I

1. Cell membrane: physiochemical properties; Molecular Organization- asymmetric
organization of lipids, proteins and carbohydrates: Biogenesis and Functions
2. Transport of small molecules across cell membranes: types and mechanisms.
3. Active transport by ATP powered pumps: types, properties and mechanisms.
4. Transport of proteins into Mitochondria and Chloroplast.

Unit II

1. Transport of proteins into and out of Nucleus.
2. Transport of proteins into Endoplasmic Reticulum.
3. Transport by Vesicle formation: Endocytosis and Exocytosis.
4. Molecular mechanisms of Vesicular transport.

Unit III

1. Intracellular digestion: Ultra structure and function of lysosomes.
2. Cell Motility and Shape I: Structure and function of microfilaments.
3. Cell Motility and Shape II: Structure and Function of Microtubules and Intermediate
4. Intra cellular communication through Cell Junctions: Occluding Junctions, Anchoring
Junctions and Communicating Junctions.

Unit IV

1. Molecular Mechanisms of Cell-Cell Adhesions: Ca++ dependent cell-cell adhesions.
2. Molecular Mechanisms of Cell-Cell Adhesions: Ca++ independent cell-cell adhesions.
3. Extracellular Matrix of animals: Organization and Functions.
4. Extracellular Matrix Receptors on animal cells: Integrins.

Unit V

1. Cell Signaling: Signaling via G-Protein linked and enzyme linked cell surface receptors,
MAP kinase pathways, Interaction and Regulation of signaling pathways.
2. Eukaryotic Cell Division Cycle: Different Phases and Molecular Events.
3. Control of Cell Division Cycle: In yeast and mammalian cells.
4. Apoptosis: Phases and significance, Morphological and Biochemical changes associated
with apoptotic cells, Apoptotic Pathways and regulators

Practical Exercises

1. Sub Cellular fractionation
2. Chromosome preparation: Mitosis-Onion root tip, rat/mouse cornea, rat mouse bone
marrow, human lymphocytes
3. Chromosome preparation: Meiosis- Rat/mouse testis, Grasshopper testis.
4. Polytene chromosome preparation from drosophila salivary gland
5. Identification of tissue typing: Histological preparation of tissue
6. Identification of different biomolecules in different tissues by histochemical techniques
7. Electron microscopy: demonstration and good photographs for interpretation

Reference Books

1. Molecular Biology of the cell (2002) Alberts, et al
2. Molecular Cell biology (2004) Lodish, et al
3. Working with Molecular Cell Biology: A study companion (2000), Stories et al
4. Cell and Molecular Biology: concepts and Experiments (3rd Ed,.2002), Gerald Karp
5. The cell: A Molecular Approach, G.M.Cooper
6. The world of Cell (1996), Becker et al
7. Cell proliferation and apoptosis (2003), Hughes and Mehnet
8. Essential Cell Biology (1998), Alberts et al
9. Biochemistry and Molecular Biology of Plants (2000) Buchanan et al
10. Harpers biochemistry Murray et al

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