Saturday, 3 March 2012

M.Sc. Biochemistry 2011-2012, semester I, cell biology , jiwaji university


101: CELL BIOLOGY

UNIT I

1. Cell Membrane: Physicochemical Properties; Molecular Organization – asymmetrical
organization of lipids, proteins and carbohydrates; Biogenesis and Functions
2. Transport of Small Molecules Across Cell Membranes: Types and Mechanism
3. Active Transport by ATP-Powered Pumps: Types, properties and mechanisms
4. Transport of proteins into mitochondria and chloroplast

UNIT II

1. Transport of proteins into and out of nucleus
2. Transport of proteins into endoplasmic reticulum
3. Transport by vesicle formation: Endocytosis and Exocytosis
4. Molecular Mechanism of vesicular transport

UNIT III

1. Intracellular Digestion: Ultra structure and Functions of Lysosomes
2. Cell Motility and Shape I: Structure and Functions of Microfilaments
3. Cell Motility and Shape II: Structure and Functions of Microtubules and Intermediate
Filaments
4. Intracellular communication through cell junctions: Occluding junctions, Anchoring Junctions
and Communicating Junctions

UNIT IV

1. Molecular Mechanism of Cell-Cell Adhesion: Ca++ dependent cell-cell adhesion
2. Molecular Mechanism of Cell-Cell Adhesion: Ca++ independent cell-cell adhesion
3. Extra-cellular Matrix of Animals: Organization and Functions
4. Extra-cellular Matrix Receptors on Animal Cells: The Integrins

UNIT V


1. Cell Signaling: Signaling via G-Protein linked and enzyme linked cell surface receptors, MAP
Kinase Pathways, Interaction and Regulation of signaling pathways
2. Eukaryotic Cell Division Cycle: Different Phases and Molecular Events
3. Control of Cell Division Cycle: In yeast and mammalian cells
4. Apoptosis: Phases and significance, Morphological and Biochemical changes associated with
apoptotic cells, Apoptotic Pathways and regulators

Practical Exercises
1. Sub cellular fractionation
2. Chromosome Preparation: Mitosis – Onion root tip, rat/mouse cornea, rat/mouse
bone marrow, human lymphocytes
3. Chromosome Preparation: Meiosis – Rat/mouse testis, Grasshopper testis
4. Polytene chromosome preparation from Drosophila salivary gland
5. Identification of tissue typing: Histological preparation of tissue
6. Identification of different biomolecules in different tissues by histochemical
techniques
7. Electron microscopy: Demonstration and good photographs for interpretation.

Reference Books


1. Molecular Biology of the Cell (2002), Alberts, et al
2. Molecular Cell Biology (2004), Lodish, et al
3. Working with Molecular Cell Biology: A study Companion (2000), Storrie et al
4. Cell and Molecular Biology: Concepts and Experiments (3rd Ed., 2002), Gerald Karp
5. The Cell: A Molecular Approach (2004), G.M. Cooper
6. The Word of the Cell (1996), Becker et al
7. Cell Proliferation and Apoptosis (2003), Hughes and Mehnet
8. Essential Cell Biology (1998), Alberts et al
9. Biochemistry and Molecular Biology of Plants (2000), Buchanan et al
10. Harpers Biochemistry Murray et al
Note: All text books are of latest editions.

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