Saturday, 3 March 2012

BACTERIOLOGY, VIROLOGY AND GENERAL MICROBIOLOGY, M.SC. (BOTANY) ,SEMESTER I, SESSION 2011 – 2013


BOT 101: BACTERIOLOGY, VIROLOGY & GENERAL MICROBIOLOGY

UNIT 1:

Bacterial taxonomy;
Identification of bacteria
General characters of Rickettsia and Chlamydia
Diseases caused by Rickettsia and Chlamydia
Mode of nutrition in bacteria; autotrophy, heterotrophy, symbiosis

UNIT II

General account of sterilization culture media, pure culture techniques;
A general idea about bacterial toxins and enzymes;
Bacteriophage;
Bacterial diseases: caused by Escherichia coli, Shigella

UNIT III

General properties and evolution of viruses;
Cultivation of virus and viral assay;
Transmission of plant viruses and control measures
Oncogeneic viruses and tumorigenesis;
Viral diseases: Encephalitis, hepatitis AIDS and Rabies

UNIT IV

Biological nitrogen fixation: symbiotic and non symbiotic nitrogen - fixation;
Fermentation technology: principle and types of fermentation
Microbial degradation of pesticides and hydrocarbons
Mycoplasmas: general account and important diseases caused by them

UNIT V

Microbial conversion of waste product with particular reference to alcohol and biogas
General account of Immunity, properties of antigens and antibodies
Allergy and types of allergies
Mycotoxins and their harmful effects

PRACTICALS 101:

1. Preparation of culture media.
2. Isolation of Bacillus and Rhizobium spp form soil and nodules.
3. Various methods of bacterial staining to study cell wall, endospore, capsule and flagella.
4. Identification of important genera by using biochemical tests: Escherichia. Azatobactor, Staphylococcus, Bacillus, Pseudomonas, Rhizobium, streptomyces, Xanthomonas.
5. Construction of bacterial growth curve.
6. Quantitative estimation of bacteria in milk.
7. Isolation of streptomycin – resistant mutants of bacteria.
8. Sensitivity test of bacteria using different antibiotics.
9. Purification of TMV and study of thermal inactivation point and dilution point.
10. Virus concentration determination by local lesion on host.
11. Study of common vectors of plant virus: Nematodes, fungi and insects.
12. Bacteriophage isolation
13. Isolation and enumeration of bacteria: Actinomycetes and fungi from soil, rhizosphere and seed using different techniques.
14. Use of selective media for isolating micro- organisms.
15. Fermentation of alcohol and biogas from waste materials (Demonstration)

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