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    BACTERIOLOGY, VIROLOGY AND GENERAL MICROBIOLOGY, M.SC. (BOTANY) ,SEMESTER I, SESSION 2011 – 2013


    BOT 101: BACTERIOLOGY, VIROLOGY & GENERAL MICROBIOLOGY

    UNIT 1:

    Bacterial taxonomy;
    Identification of bacteria
    General characters of Rickettsia and Chlamydia
    Diseases caused by Rickettsia and Chlamydia
    Mode of nutrition in bacteria; autotrophy, heterotrophy, symbiosis

    UNIT II

    General account of sterilization culture media, pure culture techniques;
    A general idea about bacterial toxins and enzymes;
    Bacteriophage;
    Bacterial diseases: caused by Escherichia coli, Shigella

    UNIT III

    General properties and evolution of viruses;
    Cultivation of virus and viral assay;
    Transmission of plant viruses and control measures
    Oncogeneic viruses and tumorigenesis;
    Viral diseases: Encephalitis, hepatitis AIDS and Rabies

    UNIT IV

    Biological nitrogen fixation: symbiotic and non symbiotic nitrogen - fixation;
    Fermentation technology: principle and types of fermentation
    Microbial degradation of pesticides and hydrocarbons
    Mycoplasmas: general account and important diseases caused by them

    UNIT V

    Microbial conversion of waste product with particular reference to alcohol and biogas
    General account of Immunity, properties of antigens and antibodies
    Allergy and types of allergies
    Mycotoxins and their harmful effects

    PRACTICALS 101:

    1. Preparation of culture media.
    2. Isolation of Bacillus and Rhizobium spp form soil and nodules.
    3. Various methods of bacterial staining to study cell wall, endospore, capsule and flagella.
    4. Identification of important genera by using biochemical tests: Escherichia. Azatobactor, Staphylococcus, Bacillus, Pseudomonas, Rhizobium, streptomyces, Xanthomonas.
    5. Construction of bacterial growth curve.
    6. Quantitative estimation of bacteria in milk.
    7. Isolation of streptomycin – resistant mutants of bacteria.
    8. Sensitivity test of bacteria using different antibiotics.
    9. Purification of TMV and study of thermal inactivation point and dilution point.
    10. Virus concentration determination by local lesion on host.
    11. Study of common vectors of plant virus: Nematodes, fungi and insects.
    12. Bacteriophage isolation
    13. Isolation and enumeration of bacteria: Actinomycetes and fungi from soil, rhizosphere and seed using different techniques.
    14. Use of selective media for isolating micro- organisms.
    15. Fermentation of alcohol and biogas from waste materials (Demonstration)