104 : TOOLS AND TECHNIQUES
UNIT I
1. Centrifugation : Basic principle, type, instrumentation and applications
2. Photometry : Basic principles of colorimetry, and UV visible spectrophotometry,
instrumentation and applications
3. Infra red spectroscopy
4. Fluorimetry : Principle, instrumentation and applications
UNIT II
1. Chromatography : Principle, types, instrumentation and applications
2. Affinity chromatography, HPLC and FPLC
3. Electrophoresis : Principle, types and applications
4. Isoelectricfocussing and isotachophoresis
UNIT III
1. Atomic absorption spectroscopy : Principle, instrumentation and applications
2. Flame emission spectroscopy : Principle, instrumentation and applications
3. Polarimetry : Principle, instrumentation and applications
4. ORD and CD
UNIT IV
1. ESR : Principle, instrumentation and applications
2. NMR, GC Mass : Basic principle, instrumentation and applications
3. X ray crystallography : Principle, instrumentation and applications
4. Radio immunoassay : Basic principle and applications
UNIT V
1. Microscopy : Light, phase contrast, interference, fluorescence and polarization microscopy
2. Electron microscopy : Principle and Applications
3. Radioactivity : Principle, Geiger Muller Counter, liquid scintillation counter, solid scintillation
counter, gamma counter
4. Autoradiography : Principles, and applications
Practical Exercises
1. Verification of Beer’s law
2. Determination of absorption maxima
3. Electrophoresis of Proteins- native and under denaturing conditions.
4. Amino acid and carbohydrate separations by paper & thin layer chromatography
5. Gas chromatography
6. Ion exchange and gel filtration chromatography
7. Separation of blood cells by density gradient centrifugation
Reference Books
1. Physical Biochemistry : Applications to Biochemistry and Molecular Biology by Freifelder
2. Biochemical Techniques : Theory and Practice by Robyt and White
3. Principles of Instrumental Analysis by Skoog and West
4. Analytical Biochemistry by Holme and Peck
5. Biological Spectroscopy by Campbell and Dwek
6. Organic Spectroscopy by Kemp
7. A Biologist’s Guide to Principles and Techniques of Practical Biochemistry by Wilson and
Goulding
8. Principles of Instrumental Analysis by Skoog, Hollar and Nicman
Note: All text books are of latest editions.
105 : Lab Course I
Consists of practical exercises listed out under 101 & 102
106 : Lab Course II
Consists of practical exercises listed out under 103 & 104
