101 CELL BIOLOGY
UNIT –I
1. Cell Membrane: Physiochemical properties; molecular organization‐asymmetric
organization of lipids, Protein and carbohydrates: Biogenesis and function.
2. Transport of small molecules across cell membranes types and mechanism.
3. Active transport by ATP powered pumps types; P‐type, V‐ type, F‐ type, ABC
transporters.
4. Properties and mechanisms of transporters; Patch clamp technique.
UNIT‐ II
1. Structure, function and transport of proteins in to mitochondria and chloroplast.
2. Transport of proteins and RNA‐into and Out of Nucleus.
3. Transport of proteins into endoplasmic reticulum and Golgi bodies.
4. Transport by vesicle formation: Endocytosis and exocytosis and molecular mechanism of
Vesicular transport.
UNIT III
1. Ultra structure and function of lysosomes, peroxysomes and Vacuoles.
2. Cell motility and shape I: structure and function of microfilaments.
3. Cell motility and shape II: Structure and function of microtubules and intermediate
filaments.
4. Intra cellular communication through cell junctions: Occluding junction, anchoring
junctions and communicating junctions.
UNIT ‐IV
1. Molecular mechanism of cell‐cell adhesions: Ca++ dependent cell‐cell adhesions.
2. Molecular mechanism of cell‐cell adhesions: Ca++ independent cell‐cell adherence.
3. Extracellular matrix of animals: organization and function.
4. Extracellular matrix receptors on animal cell: integrins.
Unit ‐V
1. Cell signaling: Signaling Via G‐Protein linked cell surface receptors, MAP kinase pathways
and tyrosine kinase pathway: Initiation, interaction and regulation.
2. Eukaryotic cell division cycle: Different phrase and molecular events.
3. Control of cell division cycle: in yeast and mammalian cells.
4. Apoptosis: Phases and significance, Morphological and Biochemical changes associated
with apoptotic cells, Apoptotic Pathways and regulators.
Practical Exercises
1. Sub Cellular fractionation
2. Chromosome preparation: Mitosis‐Onion root tip, rat/mouse cornea, rat mouse bone
marrow, human lymphocytes
3. Chromosome preparation: Meiosis‐ Rat/mouse testis, Grasshopper testis.
4. Polytene chromosome preparation from drosophila salivary gland
5. Identification of tissue typing: Histological preparation of tissue
6. Identification of different biomolecules in different tissues by histochemical techniques
7. Electron microscopy: demonstration and good photographs for interpretation
Reference Books
1. Molecular Biology of the cell (2002) Alberts, et al
2. Molecular Cell biology (2004) Lodish, et al
3. Working with Molecular Cell Biology: A study companion (2000), Stories et al
4. Cell and Molecular Biology: concepts and Experiments (3rd Ed,.2002), Gerald Karp
5. The cell: A Molecular Approach, G.M.Cooper
6. The world of Cell (1996), Becker et al
7. Cell proliferation and apoptosis (2003), Hughes and Mehnet
8. Essential Cell Biology (1998), Alberts et al
9. Biochemistry and Molecular Biology of Plants (2000) Buchanan et al
10. Harpers biochemistry Murray et al
