303: BIOPROCESS ENGINEERING AND TECHNOLOGY
UNIT I
1.Introduction to bioprocess engineering
2.Isolation, preservation and Maintenance of Industrial microorganisms.
3.Kinetics of microbial growth an death,
4.Media for industrial fermentation.
UNIT II
1. Air and media sterilization,
2. Safety in fermentation laboratory
3. Strain improvement of industrially important microorganism.
4. Bioreactors: Principle, Kinetics, types, design, and application.
UNIT III
1. Aeration and Agitation systems for bioreactor.
2. Flow behaviour of fermentation fluids
3. Gas-Liquid mass transfer, significance of KLa, and Heat transfer.
4. Automation for monitoring and control.
UNIT IV
1.Downstream processing: Introduction, removal of microbial cells and solid matter, foam
reparation, precipitation, centrifugation, cell disruption, chromatography
2.Extraction:-solvent, two phase, liquid extraction
3.Product recovery processes
4.Crystallization, packaging and quality assurance.
UNIT V
1. Safety consideration in down stream processing
2. Bioprocess economics.
3. Classification of product formation
4. Product synthesis kinetics
Practical Exercises:
1. Isolation of industrially important microbes from the environment
2. Determination of TDP & TDT of microorganisms for a design of a sterilizer
3. Determination of growth curve of a industrial organism and compute substrate,
degradation profile, specific growth rate & growth yield
4. Screening and enrichment for a primary / secondary metabolite from the environment
5. Strain improvement for higher yield of a product
6. Random and strategic screening for a metabolite
7. Media balancing experiments
8. Alcohol fermentation using different substrates and its down stream process.
Reference Books:
1. Biochemical Engineering, Aiwa S. , Humphrey A E , Millis N F. Univ. Tokyo Press,
Tokyo
2. Biochemical Reactors, Atkinson B., Pion Ltd London.
3. Biochemical Engineering Fundamentals, Bialy J E & Ollas D F. McGraw Hill Book
Cooperation New York.
4. Bioprocess Technology: Fundamentals & Applications, KTH, Stockholm.
5. Biochemical Engineering, Alba,S., Humphrey, A.E. and Millis, N.F. Univ. Tokyo Press
6. Biochemical Reactors, Atkinson, B... Pion Ltd, London
7. Biochemical Engineering Fundamentals, Bialy, J.E. and Olli’s, DF Mcgraw-Hill Book
Co. New York
8. Bioprocess Technology: Fundamentals and Applications, KTH, Stockholm
9. Process Engineering in Biotechnology, Jackson, A.T., Prentice Hall, Engelwood Cliff
10. Bioprocess Engineering : Basic concepts Shuler,M.L. and Kargi, F.Prentice Hall
Englewod Cliff
11. Principles of fermentation Technology, Stanbury, P.F. and Whitaker, A.,pergmon Press,
Oxford
12. Bioreactor Engineering Principles, Nielson,J and Villadsen, J.,Plenum Press
13. Chemical Engineering, Problems in Biotechnology, Shuler, M.L.(Ed.), AICHE
14. Bioprocess Engineering, Lee, J.M.Prentice Hall Inc.
15. Bioprocess Engineering Kinetics- , Mass Transport, Reactors and Gene Expression,
Vieth, W.F., John Wiley & Sons, Inc.
