402: MOLECULAR AND IMMUNO DIAGNOSTICS
(Optional 1)
Unit I
1. Introduction and Concept of Molecular Diagnostics
2. DNA diagnostics: PCR based diagnostics, RAPD etc
3. Southern blot & RFLP in diagnostics
4. Western blot diagnostics
Unit II
1. Genetic and chromosomal disorders: autosomal; sex chromosomal; karyotype analysis,
single cell disorders and complex traits.
2. Single nucleotide polymorphisms and diagnostic significance.
3. Nanodiagnostics
4. Microarray Technology
Unit III
1. Array-based diagnostics
2. G-banding, in situ hybridization (FISH and on-FISH), and comparative genomic
hybridization (CGH)
3. Phage display concept and applications of Phage display
4. Immunoarrays, FACs.
Unit IV
1. Immuno electrophoresis: rocket immunoelectrophoresis; CIE, Graber and William
Technique, RIA, ELISA – Principle, Methodology and applications.
2. Agglutination: Direct and Indirect, Widal test, VDRL test
3. Immuno fluorescence: Direct, indirect and Sandwich
4. Immunodiagnostics: diagnosis of infectious diseases, respiratory diseases
Unit V
2. Monoclonal antibodies in diagnosis
3. Immunoblot analysis & immunocytochemical staining.
4. Development of rapid diagnostic tests and their applications
5. Biochemical diagnostics: Biochemical markers of disease diagnosis and their
applications.
Practical Exercises: Appropriate exercises based on theory
References:
1. Molecular Diagnostics: Current Technology and Applications
by Juluri R. Rao, Colin Craig Fleming, John Edmund Moore – 2006
2. Molecular Diagnostics: A Training and Study Guide
by Gregory J. Tsongalis, William B. Coleman - 2002
3. Molecular Diagnostics: For the Clinical Laboratorian
by William B. Coleman, Gregory J. Tsongalis - 2006
4. Molecular Diagnostic PCR Handbook
by Gerrit J. Viljoen, L. H. Nel, J. R. Crowther – 2005
5. The Neuropathology of Dementia
by Margaret M. Esiri, Virginia M.-Y. Lee, John Q. Trojanowski
6. Diagnostic Bacteriology Protocols,
by Louise O'Connor - 2006
7. Immunodiagnosis of Cancer
by Ronald B. Herberman, Donald W. Mercer – 1990
8. Immunodiagnostics: A Practical Approach
by Ray Edwards - 1999
