401 : FRONTIER TECHNOLOGIES IN BIOSCIENCES
UNIT I
1. Introduction and organization of animal cell and tissue culture laboratory, Contamination,
Primary and established cell line culture
2. Serum and protein free defined media and their applications, measurement of viability and
cytotoxicity
3. Introduction to balanced salt solutions and simple growth medium : Brief introduction of the
chemical, physical and metabolic functions of different constituents of culture medium, Role
of carbon dioxide and supplements
4. Biology and characterization of the cultured cells, measurement of parameters of growth
UNIT II
1. Basic techniques of mammalian cell culture : Disaggregation of tissues, Primary and
established cell line cultures and mass culture techniques
2. Stem cell technology : Types of stem cell, manipulations of stem cells
3. Factors governing manipulation of stem cell, Therapeutic cloning for embryoinic stem cell
(ESC’s)
4. Applications of stem cell cultures
UNIT III
1. Introduction of plant tissue culture and laboratory organization
2. Tissue culture media (composition and preparation), initiation and maintenance of callus
and suspension culture, single cell clones
3. Organogenesis : Somatic embryogenesis, transfer and establishment of whole plants in soil
4. Protoplast isolation, culture, regeneration and somatic hybridization and applications of
plant tissue culture
UNIT IV
1. Nanobiotechnology : Introduction to nanoscinece, Tools for measuring nano structures
2. Biosensor development and its applications
3. Microarray chips : Types of DNA chips and their production
4. SNP’s and GMS (Genome mismatch repair)
UNIT V
1. Functional proteomics : Methods pf proteome analysis
2. Human Genome Project (HGP) : The human genome/ Social implications
3. Forensic applications of DNA analysis
4. Patents and intellectual property : Intellectual property areas : Trademarks, Copyrights. The
process of obtaining a patent, Why obtain a patent, Recent changes in IPR and Patent
policies
Reference Books:
1. DNA Microarrays and Gene Expression by P.Baldi and G.W.Hatfield
2. Protein-Protein Interactions by Erica Golemis
3. A passion for DNA (Genesm Genomes and Society) by J.D.Watson
4. Modern Genetic Analysis by Anthny J.F. Griffiths et al.
5. Nanobiotechnology- Next Big Idea by Mark, Ratner, Daniel Ratner
6. Gene Cloning by T.A.Brown
7. Latest information on academic Websites
Note: All text books are of latest editions.
