204: Part B . ANIMAL BIOTECHNOLOGY
UNIT IV
1. Introduction and organization of animal cell and tissue culture laboratory
2. Primary and established cell line cultures
3. Serum and protein free defined media and their applications
4. Introduction to balanced salt solutions and simple growth medium: rationale of
composition of medium, role of CO2 and supplements
UNIT V
1. Measurement of parameters of growth
2. Scaling up of animal cell culture, Cell synchronization
3. 3‐D animal cell culture
4. FISH and applications of animal cell culture
Practical Exercises: Part B
1. Preparation of tissue culture medium and membrane filtration
2. Preparation of single cell suspension from spleen and thymus
3. Cell counting and viability
4. Macrophage monolayer from PEC and measurement of phagocytic activity
5. Cell fusion with PEG
Reference Books
1. Culture of Animal Cells by RI Freshney
2. Animal Cell Culture: Practical Approach John R W Masters
3. Animal Cell Culture Techniques by Ed. Martin Clynes
4. Methods in Cell Biology Vol. 57, Animal cell culture methods
