EC-103: Spectro-analytical Methods of Analysis-I
M.M.:35
Unit-I: Colorimetry and Spectrophotometry
An introduction to spectrophotometric methods, a brief idea of wave properties of electromagnetic radiation, theory of spectrophotometry and colorimetry, limitations of Beer's Law, classification of methods of colour measurement, instrumentation single beam and double beam, photometric error, applications of spectrophotometry to inorganic and organic compounds (quantitative calculations), near IR spectrophotometry, spectrophotometric titration.
Unit-II: Other Spectro-analytical techniques
(A) Introduction, general principle, instruments for nephelometry and tubidimetry, applications of nephelometry and turbidimetry to analytical chemistry.
(B) Dispersion Refrectometry and Flame photometry
(C) Polarometry, circular dichrosim (CD) and optical rotatory dispersion (ORD).
Unit-III: Emission Spectroscopy
Elementary idea of emission spectroscopy, introduction, elementary theory, instrumentation, types of flames, interferences, factors affecting flame photometry, applications to qualitative and quantitative analysis, limitations.
Unit-IV: Fluorescence and Phosphorescence Spectrophotometry
Theory of fluorescence and phosphorescence, quantum yield, factors affecting fluorescence and phosphorescence, relation between concentration and intensity, instrumentation, applications, an elementary idea of chemiluminiscence.
Unit-V: Kinetic of Slow and Fast reactions (An elementary study keeping in view its applications in analytical chemistry)
(A) Rates of chemical reaction, expression for reaction rate, rate constants, order of reaction, methods for determination of order of reaction, Arrhenius equation, Collision theory, failure of collision theory, Absolute reaction rate theory, unimolecular reactions, mathematical formulation of Lindemann’s theory, catalysed reactions, Theory of homogenous catalysed reactions, kinetics of enzyme catalysed reactions, elementary idea of micellar catalysis.
(B) Study of fast reactions by stopped flow method, relaxation methods, flash photolysis method, photochemical reactions, kinetics of photochemical combination of hydrogen and chlorine, branched chain reactions, oscillatory reactions, applications of kinetic methods in finding out optimum conditions for different reactions.
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Books Recommended
1. John. R. Dyer, Applications of Absorption Spectroscopy of Organic compounds,9th ed.. Prentice Hall of
India Pvt. Ltd. (1994).
2. Dudley H.Williams and Ian Fleming, Spectroscopic Methods in Organic Chemistry, 4th ed., Tata Mc- Graw Hill Book Company (1998).
3. R.M.Silverstein, G.Clayton Bassler and Terence C. Morrill, Spectroscopic Identification of Organic
compounds, 6th ed, John Wiley & Sons (1998).
4. D.A. Skoog, F.J. Holler and Nieman, Principles of Instrumental Methods, 5th ed.,Thomson Asia Pvt.
Ltd., Singapore (2003).
5. R.A. Day and A.L. Underwood, Quantitative Analysis, 6th ed., Prentice Hall of India Pvt. Ltd. (1993).
6. G.D. Christian., Analytical Chemistry, 6th ed, John Wiley & Sons (2000)
7. A.I. Vogel, Textbook of Quantitative Chemical Analysis, 5th ed., Addison Wesley Longman
Singapore (1999)
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