EC -202: Spectro-analytical Methods of Analysis- II
M.M.: 35
Unit-I: Atomic Absorption and Emission Spectroscopy.
Theory of atomic spectroscopy, the origin of spectral transition, the populations of energy levels, the factors influencing spectral width, atomic absorption spectroscopy, instrumentation, interferences, applications, various non-flame emission sources, applications, comparison of atomic emission and atomic absorption methods.
Unit-II: Infrared Spectroscopy
Theory of Infrared absorption, vibrational modes, vibrational coupling, instrumentation, dispersive and non-dispersive instrument, qualitative applications and interpretation of spectra, quantitative applications. A brief idea of Raman spectroscopy.
Unit-III: Nuclear Magnetic Resonance Spectroscopy
Theory of NMR, chemical shift and spin-spin splitting, relaxation process of saturation, environmental effects on NMR spectra, instrumentation, CW or FT NMR instrument, Rules governing the interpretation of first order spectra, applications to quantitative analysis.
Unit-IV: Carbon-13 NMR
Historical development, proton decoupling-broad band, Off-resonance and pulsed or gated decoupling, nuclear overhauser enhancement, polarization transfer experiments-DEPT and INEPT chemical shifts, spin-spin coupling impacts, application of 13C NMR to structure determination, two-dimensional NMR spectroscopy, principle, the COSY experiment, the COSY experiment with double quantum filter (COSY-DQF), the NOESY experiment, three-dimensional NMR experiment.
Unit-V: Mass Spectrometry
Theory of mass spectrometry, practical considerations, ion production, depletion of ions, ion detector, calibration, other ionization techniques: chemical ionization, fast atom bombardment (FAB), and electrospray, interpretation of the mass spectrum of the compound, applications of mass spectrometry.
Books Recommended
1. John. R. Dyer, Applications of Absorption Spectroscopy of Organic compounds, 9th ed.,Prantice Hall of
India Pvt. Ltd. (1994).
2. Dudley H.Williams and Ian Fleming, Spectroscopic Methods in Organic chemistry, 4th ed., Tata Mc-Graw Hill Book company (1998).
3. R.M.Silverstein, G.Clayton Bassler, and Terence C. Morrill, Spectroscopic Identification of Organic
Compounds, 6th ed., John Wiley & Sons (1998).
4. C.N. Ban well, Fundamentals of Molecular Spectroscopy, Tata Mc-Graw Hill Book company (1998).
5. Manas Chanda, Atomic Structure and Chemical Bond, Tata Mc-Graw Hill Book company (1998).
