BCH 101: Fundamentals of Cell Biology
UNIT I
1. Ceilular and Chemical Foundations of Life
2. Prokaryotic and Eukaryotic cells: Comparative Study; Cells as Experimental Models
3. Cell Membrane: Physicochemical Properties; Molecular Organization - asymmetrical organization of
lipids, proteins and carbohydrates; and Functions
4. Molecular Models and Biogenesis of Cell Membrane
UNIT II
L. Transport of Small Molecules Across Cell Membranes: Types and Mechanism
2. Active Transport by ATP-Powered Pumps: Types, properties and mechanisms
3. Transport of proteins into mitochondria and chloroplast
4. Transport of proteins into and out of nucleus
UNIT III
L. Transport of proteins into endoplasmic reticulum
2. Processing of Proteins in Endoplasmic Reticulum and GolSi Apparatus
3. Transport by vesicle formatlon: Endocytosis and Exocytosis
4. Molecular Mechanism of vesicular transport
UNIT IV
1. lntracellular Digestion: Ultra structure and Functions of Lysosomes
2. Peroxisomes: Ultra structure, Functions and Biogenesis
3. Cell Motility and Shape l: Structure and Functions of Microfilaments
4. Cell Motility and Shape lt: Structure and Functions of Microtubules and lnterrnediate Filaments
UNIT V
1. lntracellular communication through cell junctions: Molecular structure, organization and functions of
Occluding Junctions, Anchoring lunctions and Communicating J unctions
2. Molecular t\4echanism of Cell-CellAdhesion: Ca**dependent and Ca*. independent cell-cell adhesion
3. Extra-cellular Matrix of Animals: Molecular Composition, organization and Functions
4. Extra-cellular Matrix Receptors on Animal cells: Molecular Structure, Types and Distribution of lntegrins
Practical Exercises
Sub cellular fractionation
Chromosome Preparation: Mitosis - Onion root tip, rat/mouse cornea, rat/mouse bone marrow, human
lymphocytes
Chromosome Preparation: Meiosis - Rat/mouse testis, Grasshopper testis
Polytene chromosome preparation from Drosophila salivary gland
identification of tissue typing: Histological preparation of tissue
BCH 102: BIOMOTECULES
UNIT I
1. Classification of carbohydrates, structure and function of monosaccharides and disaccharides:
2. Structure and function of homo- and hetero-polysaccharides
3. Glycoconjugates: Proteoglycans,clycoproteins,Glycolipids: structure and biological roles.
4. Ca rbohydrates as informationalmolecules
Unit ll
1. Lipids: Types, structure, properties and biological roles; Fatty acids: Structure, types and properties
2. Saponifiable lipids: Types, structure and properties
3. Non saponiofiable lipids : Structure, types and properties
4. Lipoproteln, lipopolysaccarides and other hybrid molecules: Types, properties and functions
uNtT
1. Amino acids: Structure, classification, abbreviations, properties and functions
2. Peptides and polypeptides
3. Synthesis of peptides and protein sequencing
4. Proteins: Properties, covalent structure, secondary, tertiary and quaternary structure
UNIT IV
1. Enzymes : Classification, mechanism of action, allosteric enzymes, multienzyme coinplex
2. Enzyme kinetics: Basic concepts
3. Water soluble vitamins : Structure, distribution, interaction and biologicalfunctions (mechanism of
action not included)
4. Fat soluble vitamins :Structure, distribution and functions
UNIT V
1. Nucleotides : Structure of purine and pyrimidine bases, nucleosides, nucleotides
2. DNA: Structure and Conformation
3. DNA: denaturation, degradation, modification, repair, recombination and rearrangement
4. RNA:Structure, types and functions
Practical Exercises
1 Titration of amino acids
2 Colorimetric determination of pKa
3 Model building using space filling/ ball and stuck models
4 Reaction of amino acids, sugars and lipids
5 Quantitation of proteins and sugars
6 Analysis of oils : iodine number, saponification value, acid number
6. ldentification of different biomolecules in different tissues by histochemical techniques
7. Electron microscopy: Demonstration and good photographs for interpretation
Reference Books
1. Molecular Biology of the Cell, Alberts, et o/
2. Molecular Cell Biology, Lodish, et al
3. Working with Molecular Cell Biology: A study Companion, Storrie et o/
4. Cell and Molecular Biology: Concepts and Experiments, Gerald Karp
5. The Cell: A Molecular Approach, G.M. Cooper
6. The Word of the Cell, Becker et o/
7. Cell Proliferation and Apoptosis, Hughes and Mehnet
8. Essential Cell Biology, Alberts et o/
9. Biochemistry and Molecular Biology of Plants, Buchanan ef o/
10. Harpers Biochemistry Murray ef o/
Nofe: All text books are of latest editions.
BCH 103: MICROBIAL BtOCHEMISTRY
Unit 1
classification of Microorganisms: Basis of microbial classification, Haekel three kingdom,
Whitta ker's five kingdom concept.
Morphology and fine structure of eubacteria and archeobacteria cell wall, cytoplasmic membrane and
other organelles.
Pure culture techniques and preservation methods.
Preparation of Culture media, microbial staining.
Unit 2
Sterilization: Physical and chemical methods
Microbial Growth: Bacterial growth curve, Mathematical expression, measurement of Growth and
factors affecting growth
Microbial Nutrition: Nutritional classification of Microorganisms, common nutritional requirements,
mode of nutrition, transport of nutrients across the bacterial membrane
oxygen toxicity: study of cata lase, peroxidase, superoxide dism utase, mecha nism of oxygen toxicity.
Unit 3
Virus: Types, lsolation, cultivation, identification and viral replication.
Structure and morphology of Bacteriophage, Lytic and lysogenic cycle.
Life cycle of DNA Viruses: SV 40, RNA Viruses: Retroviruses
Cynobacteria : Generalaccount and their importance
Unit 4
1 lnfection and dlsease, types of lnfection, Mechanism of pathoBenicity 2 Bacterial Diseases: Staphylococcal and Clostridialfood poisoning, Salmonellosis Shigellosis 3 Fungaldiseases:Histoplasmosis,Aspergillosis
4 Viraldiseases: Chicken pox, Hepatitis B, and poliomyelitis
UNITV
Mycoplasmas and diseases caused by them
Bacterial Recombination:Transformation, conjugation, Transduction, plasmids and transposons
Chemotherapeutlc agents: Classification of antibiotics, Broad spectrum antibiotics, Antibiotics from
prokaryotes
Anti-fungal and antiviral antibiotics, mode of action of antibiotics and resistance to antibiotics
Practical Exercises
1 Preparation of liquid and solid media for growth of microorganisms. 2' lsolation and maintenance of organisms by plating, streaking and serial dilution methods. Slants and
stab cultures. Storage of microorganisms
lsolation of pure cultures from soil and water
Refrences
Principles of Biochemistry by Nelson, Cox and Lehninger
Biochemistry by G.Zubay
Biochemistry by Stryer
Biochemistry by Garrett and Grisham
Biochemical Calculations, lrwin H. Seigel, John Wiley and Sons lnc'
Biochemistry, Dvoet and JG. Voet, J Wiley and Sons'
Biochemistry, D Frelfilder, W.H. Freeman & Company'
Laboratory Techniques In Biochemistry and molecular Biology, Work and Work
A Biologisi,s guide to principles and Techniques of Practical Biochemistry, Wilson & GouldinS, ELBS
Edition
