EC-101: Fundamentals of Quantitative Analysis and Separation Methods
Unit-I: Concepts involved in Analysis
Role of analytical chemistry, classification of analytical methods-classical and instrumental, types of instrumental analysis, selecting analysis method, neatness and cleanliness, laboratory operations and practices, good laboratory practices, techniques of weighing, errors, volumetric glassware-cleaning and calibration of glassware, sample preparation – dissolution and decompositions, selecting and handling reagents, laboratory notebooks, safety in the analytical laboratory, calibration and detection limits, proficiency testing.
Unit-II: Titrimetric and Gravimetric Methods of Analysis
General principles: Solvents in analytical chemistry, acid-base equilibria, concentration systems, stoichiometic calculation, acid-base titration, titration curves, acid base indicators, applications of acid-base titration, complexometric titration, metal-ion indicators, precipitation titration, Mohr’s titration, Volhard’s titration, adsorption indicators, Fajan’s titration, titration curves in oxidation-reduction titration, redox indicators, applications of redox titrations.
Unit-III: Separation Techniques -I
(A) Solvent Extraction: Fundamental treatment, theoretical principle, classification, and factors favouring extraction, extraction equillibria, applications.
(B) Solid phase extraction and solid phase micro extraction, applications.
(C) Ion- Exchange: Theories, use of synthetic ion exchange in separation, chelating ion exchange resins, liquid ion exchangers, experimental technique.
Unit-IV: Separation Techniques -II
An introduction to chromatographic methods, paper, thin layer and column chromatography, theory of chromatography, classification of chromatographic techniques, retention time, relationship between retention time and partition coefficient, the rate of solute migration, differential migration rates, band broadening & column efficiency, kinetic variables affecting band broadening, Electrophoresis and capillary electrophoresis.
Unit-V: GC and HPLC
Instrumentation of GC and HPLC, applications in qualitative and quantitative analysis, comparison of GC and HPLC, Ion chromatography, pyrolytic gas chromatography, size exclusion chromatography, super critical fluid chromatography, affinity chromatography.
Books Recommended
1. D.A. Skoog, F.J. Holler and T.A. Nieman, Principles of Instrumental Methods, 5th ed.,,Thomson Asia Pvt. Ltd., Singapore (2003).
2. R.A. Day and A.L. Underwood, Quantitative Analysis, 6th ed., Prentice Hall of India Pvt. Ltd
(1993).
3. G.D. Christian, Analytical Chemistry, 6th ed, John Wiley & Sons (2001).
4. S.M. Khopker, Environmental Pollution Analysis, 2nd ed., New Age International
Pvt. Ltd.(2002).
5. A.I. Vogel, Textbook of Quantitative Chemical Analysis, 5th ed., Addison Wesley Longman
Singapore (1999) 1
6. G. W. Eving, Instrumental Methods of Chemical Analysis, 5th ed.,Mc-Graw Hill Book
Company (1985).
7. Willard, Merritt, Dean, and Settle, Instrumental Methods of Analysis, 7th ed., C B S
Publishers &Distributors (1986).
