203: MICROBIAL & ANIMAL BIOTECHNOLOGY
Part A: Microbial Biotechnology
UNIT I
1. Microbial of Industrial Importance
2. Microbial Production of antibiotics: Penicillin; and Streptomycin
3. Microbial Production of Vitamins & amino acids (Vit B12 & Glutamic acid)
4. Microbial production of enzymes: Amylase, Protease and applications
UNIT II
1. Microbial production of alcoholic beverages: Distilled alcoholic beverages-Beer,
microbial production of Vinegar.
2. Microbial production of Organic acids: Citric acid & Acetic acid
3. Microbial production of solvents: Ethanol and acetone
4. Microbial production of food –SCP
UNIT III
1. Biotransformation: Steroids
2. Mushroom Cultivation
3. Biofertilizers and its applications
4. Immobilization of microbial cells and their applications
Practical Exercises in Part A
1. Isolation of Industrially important microorganisms for microbial processes
2. Microbial production of citric acid using Aspergillus niger
3. Production and estimation of Alkaline protease
4. Biomass SCP from Algae and Fungi
5. Organic Solvent production
6. Bio-transformation
7. bio-insecticide isolation, purification and assay
8. Bio-fertilizer production
9. Use of alginate for cell immobilization
Part B Animal Biotechnology
UNIT IV
1. Introduction and organization of animal cell and tissue culture laboratory
2. Primary and established cell line cultures
3. Serum and protein free defined media and their applications
4. Introduction to balanced salt solutions and simple growth medium: rationale of
composition of medium, role of CO2 and supplements
Part B Animal Biotechnology
UNIT IV
1. Introduction and organization of animal cell and tissue culture laboratory
2. Primary and established cell line cultures
3. Serum and protein free defined media and their applications
4. Introduction to balanced salt solutions and simple growth medium: rationale of
composition of medium, role of CO2 and supplements
UNIT V
1. Measurement of parameters of growth
2. Scaling up of animal cell culture, Cell synchronization
3. 3-D animal cell culture
4. FISH and applications of animal cell culture
Practical Exercises in Part B
1. Preparation of tissue culture medium and membrane filtration
2. Preparation of single cell suspension from spleen and thymus
3. Cell counting and viability
4. Macrophage monolayer from PEC and measurement of phagocytic activity
5. Cell fusion with PEG
Reference Books
1. Culture of Animal Cells by RI Freshney
2. Animal Cell Culture: Practical Approach John R W Masters
3. Animal Cell Culture Techniques by Ed. Martin Clynes
4. Methods in Cell Biology Vol. 57, Animal cell culture methods
