102 : BIOMOLECULES
UNIT I
1. Carbohydrates : Structure, classification, properties and functions
2. Home and heterpolysaccharides : carbohydrate derivatives
3. Lipids : Classification, structure, properties and functions
4. Lipids with special biological functions
UNIT II
1. Amino acids : Structure, classification, abbreviations, properties and functions
2. Peptides and polypeptides
3. Synthesis of peptides and protein sequencing
4. Proteins : Properties, covalent structure, secondary, tertiary and quaternary structure
UNIT III
1. Enzymes : Classification, mechanism of action, allosteric enzymes,
multienzyme complex
2. Enzyme kinetics : Basic concepts
3. Water soluble vitamins : Structure, distribution, interaction and biological functions
(mechanism of action not included)
4. Fat soluble vitamins : Structure, distribution and functions
UNIT III
1. Nucleotides : Structure of purine and pyrimdine bases, nucleosides, nucleotides
2. DNA : Structure and Conformation
3. DNA : denaturation, degradation, modification, repair, recombination and rearrangement
4. RNA : Structure, types and functions
UNIT V
1. Animal hormones : Structure and biological roles
2. Plant hormones : Structure and biological functions
3. Plant phenolics: Classification and functions
4. Alkaloids : Classification and functions
Practical Exercises
1. Titration of amino acids
2. Colorimetric determination of pKa
3. Model building using space filling/ ball and stuck models
4. Reaction of amino acids, sugars and lipids
5. Quantitation of proteins and sugars
6. Analysis of oils : iodine number, saponification value, acid number
Refernce Books
1. Principles of Biochemistry by Nelson, Cox and Lehninger
2. Biochemistry by G.Zubay
3. Biochemistry by Stryer
4. Biochemistry by Garrett and Grisham
5. Biochemical Calculations, Irwin H. Seigel, John Wiley and Sons Inc.
6. Biochemistry, DVoet and JG. Voet , J Wiley and Sons.
7. Biochemistry, D Freifilder, W.H. Freeman & Company.
8. Laboratory Techniques in Biochemistry and molecular Biology, Work and Work
9. A Biologist’s guide to Principles and Techniques of Practical Biochemistry,
Wilson & Goulding, ELBS Edition.
Note: All texts are of latest editions.
